AUTONOMIC CONTROLS OF BODY WEIGHT AND FEEDING

拟议的项目继续对神经进行程序性调查。 控制摄食和体重调节的基本机制。 该项目的长期目标是一个全面的功能 动态平衡神经解剖学。在不久的将来，这个目标需要 迷走神经的一系列结构-功能分析的形式。 广泛的行为和生理证据最近证实 迷走神经，第X脑神经，在食物中起着重要作用 摄入量和身体能量调节。然而，目前仍在继续取得进展 在分析与进食行为有关的迷走机制和 生理学严重受阻于缺乏关于 迷走神经脑干核团的组织、迷走神经的分布 消化道和腹部器官中的纤维，以及 前脑和脑干控制不同迷走神经的机制 外流协调消化和能量处理。 因此，该提案的近期目标是完成一系列 在上一阶段开始的有希望的分析，这些分析更加全面 描述该组织的地形和其他组织原则 副交感神经流出中的最后三个神经元中继(即 节后、节前和节前传入) 控制内脏器官的消化代谢。两大 一系列实验旨在为以后的更多 提出了聚焦功能分析：第一组将产生 节后靶点的单独清单、分布 模式，以及不同池的端子配置文件类型 迷走神经背侧运动核内的运动神经元。此外， 将绘制迷走神经传入在腹部器官中的分布图 同时。第二组将开始描绘不同的中枢神经系统 传入投射，并由本地电路控制 已确定的运动神经元池内的背侧运动核团 迷走神经。这两组实验都将采用高度选择性的迷走神经切断术， 使用神经示踪剂进行双重和三重标记，并具有高分辨率 定量显微镜策略，以及生理分析。 概述的课程将扩展我们对主要神经功能的理解 与代谢和消化系统疾病有关的机制包括 肥胖，糖尿病，厌食症，吞咽障碍，迷走神经功能障碍， 消化性溃疡和饮食失调。

The proposed project continues a programmatic investigation of the neural mechanisms underlying the control of feeding and body weight regulation. The long-range goal of the project is a comprehensive functional neuroanatomy of homeostasis. For the immediate future, this goal takes the form of a series of structure-function analyses of the vagus verve. Extensive behavioral and physiological evidence has recently established that the vagus, the Xth cranial nerve, is importantly involved in food intake and body energy regulation. Currently, however, continued progress in analyzing the vagal mechanisms implicated in ingestive behavior and physiology is seriously hampered by a lack of information about the organization of the vagal brainstem nuclei, the distribution of the vagal fibers in the digestive tract and abdominal organs, and the architecture of the forebrain and brainstem mechanisms controlling the different vagal outflows coordinating digestion and energy handling. Hence, the immediate goal of the proposal is the completion of a series of promising analyses, begun during the last period, that are more fully characterizing the topographic and other organizational principles of the last three neuronal relays in the parasympathetic outflow (i.e. the postganglionics, preganglionics, and afferents to the preganglionics) controlling the visceral organs of digestion and metabolism. Two major sets of experiments designed to provide a foundation for later, more focused functional analyses are proposed: The first set will generate separate inventories of the postganglionic targets, the distribution patterns, and the types of terminal profiles for the different pools of motor neurons in the dorsal motor nucleus of the vagus. Additionally, the distributions of the vagal afferents in the abdominal organs will be mapped simultaneously. The second set will begin to delineate the different CNS afferent projections to, and the local circuitry controlling, the identified pools of motor neurons within the dorsal motor nucleus of the vagus. Both sets of experiments will employ highly selective vagotomies, double and triple labeling with neural tracers, and high resolution quantitative microscopy strategies, as well as physiological analyses. The program outlined will extend our understanding of major neural mechanisms implicated in metabolic and digestive diseases including obesity, diabetes, anorexia, disorders of swallowing, vagal dysfunction, peptic ulcers, and eating disorders.