CAMPYLOBACTER PYLORIDIS IN GASTRODUODENAL DISEASE

幽门螺杆菌在胃中的存在与 有消化性溃疡、非溃疡性消化不良和胃炎。 细菌培养或组织学检测 检查需要侵入性的内窥镜检查程序 已经排除了大规模研究的可能性。 我们已经开发并验证了一种可靠的非侵入性呼吸 基于幽门螺杆菌内源尿素酶含量较高的检测。 这项测试使用13C-尿素，并检测幽门螺杆菌的存在 出现13CO2引起的胃部感染 呼吸。该测试还测量了体内的抗生素敏感性。 幽门螺杆菌及其对药物治疗的反应。我们建议 研究流行病学，病理学，分子生物学， 幽门螺杆菌的免疫学和微生物学。呼气测试 将促进对猪瘟血清流行病学的快速经济研究 幽门螺杆菌感染。这些研究包括：1)患病率 无症状个体感染幽门螺杆菌的情况；2) 十二指肠胃溃疡患者幽门螺杆菌的感染情况 溃疡、非溃疡性消化不良和慢性复发性儿童 腹痛；3)幽门螺杆菌的家庭传播 4)解剖范围与解剖范围的关系。 胃幽门螺杆菌感染对胃炎的严重程度；5) 幽门螺杆菌感染与慢性萎缩性胃炎发生发展的关系 6)幽门螺杆菌感染水平与胃炎的关系 十二指肠溃疡疾病的复发和7)发展 永久根除幽门螺杆菌的治疗方案。 最后，我们将评估根除C. 幽门螺杆菌对胃组织学、十二指肠溃疡复发和 论非溃疡性消化不良症状的缓解。这个 临床研究将提供机会来解决基本的 问题：是否有个别的幽门螺杆菌菌株，血清型， 质粒，或与特定基因相关的DNA杂交组 疾病进程？我们还将比较同种类型的性质 幽门螺杆菌感染的独特型抗体反应。一个 将开发ELISA法来鉴定那些免疫显性的 幽门螺杆菌现有或过去抗原表位的研究 幽门螺杆菌感染。最后，我们将描述 幽门螺杆菌致病毒力因子及致病机制。

The presence of C. pyloridis in the stomach has been associated with peptic ulcer disease, non-ulcer dyspepsia and gastritis. Detection of the organism by either bacterial culture or histologic examination has required invasive endoscopic procedures which have precluded large scale studies. We have developed and validated a reliable noninvasive breath test based on the high endogenous urease content of C. pyloridis. This test uses 13C-urea and detects the presence of C. pyloridis infection in the stomach by the appearance of 13CO2 in the breath. The test also measures the in vivo antibiotic sensitivity of C. pyloridis and its response to drug treatment. We propose to study the epidemiology, pathology, molecular biology, immunology, and microbiology of C. pyloridis. The breath test will facilitate rapid economical studies of the seroepidemiology of C. pyloridis infections. These studies include: 1) the prevalence of C. pyloridis infection in asymptomatic individuals; 2) the prevalence of C. pyloridis in patients with gastric ulcers, duodenal ulcers, non-ulcer dyspepsia and children with chronic recurrent abdominal pain; 3) the transmission of C. pyloridis among family members; 4) the relationship of the anatomical extent and severity of C. pyloridis infection in the stomach to gastritis; 5) the relationship of C. pyloridis to development of chronic atrophic gastritis; 6) the relationship between C. pyloridis levels and relapse of duodenal ulcer disease and 7) the development of therapeutic regimens that permanently eradicate C. pyloridis. Finally, we will evaluate the consequences of eradicating C. pyloridis on gastric histology,, on relapse of duodenal ulcers and on obtaining symptomatic relief in non-ulcer dyspepsia. The clinical studies will provide the opportunity to address the basic questions: are there individual C. pyloridis strains, serotypes, plasmids, or DNA hybridization groups associated with specific disease processes? We will also compare the nature of the isotype and idiotype antibody response to C. pyloridis infection. An ELISA assay will be developed to identify those immunodominant epitopes of C. pyloridis antigens arising from current or past infection by C. pyloridis. Finally, we will characterize the virulence factors and mechanisms of C. pyloridis pathogenesis.