GENETIC REGULATION OF PHOSPHOLIPID SYNTHESIS IN YEAST

将对磷脂合成的调节进行分析 单细胞真核生物，酿酒酵母。这些研究 将涉及分离和鉴定具有缺陷的突变体 磷脂的合成和/或调节。变种人将被雇佣 在监管研究中，它们将被用于促进克隆 编码磷脂膜相关酶的结构基因 生物合成。克隆的基因将被用于研究 外源前体对磷脂合成的调节作用 如肌醇和胆碱。克隆的基因还将用于 促进将这些蛋白质组装成其特定的 在细胞膜上的位置。 人们对真核细胞控制细胞生长的机制知之甚少 与正在进行的膜配合合成膜脂 生物发生学。然而磷脂，特别是含有肌醇的磷脂 磷脂参与了复杂的信号转导过程， 在高等医学中发挥控制细胞生长和增殖的作用 真核生物。酵母细胞合成一种典型的真核混合物 磷脂，使用与高等磷脂相似的途径 真核生物。生物体在基因上是容易驯服的，并且可以被操纵 利用强大的分子遗传学。因此，酿酒酵母呈现出一种 不同寻常的机会全面了解 膜过程中磷脂合成的调控机制 简单真核生物的生物发生和细胞生长。

An analysis of regulation of phospholipid synthesis will be conducted in the unicellular eukaryotic organism, Saccharomyces cerevisiae. The studies will involve isolation and characterization of mutants with defects in the synthesis and/or regulation of phospholipids. The mutants will be employed in regulatory studies and they will be used to facilitate the cloning of structural genes encoding membrane associated enzymes of phospholipid biosynthesis. The cloned genes will be employed in studies of the regulation of phospholipid synthesis in response to exogenous precursors, such as inositol and choline. The cloned genes will also be used to facilitate studies of the assembly of these proteins into their specific locations in the cellular membranes. Little is known about the mechanisms by which eukaryotic cells control the synthesis of membrane lipids in coordination with ongoing membrane biogenesis. Yet phospholipids, particularly inositol containing phospholipids have been implicated in complex signalling processes which play a role in controlling cell growth and proliferation in higher eukaryotes. Yeast cells synthesize a typically eukaryotic mixture of phospholipids, using pathways which are similar to those in higher eukaryotes. The organism is genetically tractable, and can be manipulated using a powerful molecular genetics. Therefore, S. cerevisiae presents an unusual opportunity to develop a comprehensive understanding of the regulatory mechanisms controlling phospholipid synthesis during membrane biogenesis and cell growth in a simple eukaryote.