REGULATION OF GENE EXPRESSION BY ETHYLENE

植物激素乙烯对人体健康有深远影响。 高等植物组织器官的生长和分化。 为了阐明乙烯的作用机制，分子和 调控乙烯诱导基因表达的细胞事件 将会被定义。具体地说：1)在遗传上 对乙烯的反应将被确定。这将会实现的 通过检测由乙烯编码的mRNA的积累- 用原位杂交技术研究特定细胞中的可诱导基因 技巧。2)控制的DNA调控元件 将定义乙烯诱导的基因表达。这将是 通过在体外改变克隆的DNA序列来完成 乙烯诱导基因。植物将被改造成 诱变基因及其损伤对基因表达的影响 将会被评估。3)与细胞因素相互作用 克隆的乙烯诱导基因将被分离出来。这将是 通过分离特定结合的核蛋白来完成 与DNA调控序列相关联。来自这些的结果 实验将提供关于调节的重要线索 乙烯的基因表达及其作用机制 植物发育过程中的激素作用。

The plant hormone ethylene has a profound influence on the growth and differentiation of tissues and organs in higher plants. To elucidate the mechanism of ethylene action, the molecular and cellular events which regulate ethylene-inducible gene expression will be defined. Specifically: 1) The cell types that genetically respond to ethylene will be determined. This will be accomplished by detecting the accumulation of mRNAs encoded by ethylene- inducible genes in specific cells using in situ hybridization techniques. 2) The DNA regulatory elements that control ethylene-inducible gene expression will be defined. This will be accomplished by altering in vitro the DNA sequence of cloned ethylene-inducible genes. Plants will be transformed with the mutagenized genes and the effect of the lesion on gene expression will be assessed. 3) The cellular factors that interact with the cloned ethylene-inducible genes will be isolated. This will be accomplished by isolating nuclear proteins that specifically bind to the DNA regulatory sequences. Results from these experiments will provide important clues about the regulation of gene expression by ethylene and the mechanism of ethylene hormone action during plant development.