NMR PULSE SHAPING FOR BIOMOLECULES AND IMAGING

此前已有研究表明，射频脉冲整形可以 增强的磁共振图像和改进的抑制 水信号在小分子核磁共振波谱中的应用 溶液中的分子。我们建议将这项技术扩展到 研究胰高血糖素，其聚集动力学类似于蛋白质 折叠，并研究肌动蛋白：Profilin复合体，它扮演着一个 在肌肉收缩中起中心作用。我们将修改现有的 商用高场核磁共振将使脉冲整形成为常规 可用。我们还将通过合作努力，展示 提高了傅里叶变换的带宽并减少了放大器的停滞时间 变换电子顺磁共振。最后，我们生病了 继续我们的计算努力以开发更有用的形状， 并应用一种新的理论形式主义来量化 对进一步改进方向的预测。

Radiofrequency pulse shaping has previously been shown to give enhanced magnetic resonance images and improved suppression of the water signal in nuclear magnetic resonance spectroscopy of small molecules in solution. We propose to extend this technique to study glucagon, whose aggregation dynamics are analogous to protein folding, and to study the actin:profilin complex, which plays a central role in muscle contraction. We will modify an existing commercial high field NMR to make pulse shaping routinely available. We will also, in a collaborative effort, demonstrate improved bandwidth and reduced amplifier dead time for Fourier transform electron paramagentic resonance. Finally, we ill continue our computational efforts to develop more useful shapes, and apply a new theoretical formalism to make quantitative predictions about directions for further improvement.