CONTROL OF TRANSCRIPTION TERMINATION IN E. COLI

对十“率噬菌体的研究在 生成有关通用监管机制的信息。 转录终止系统对基因表达的调控 -首先在噬菌体中描述的抗终止药物，现在已经在 真核细胞，特别是c-myc和HIV基因的调控 表情。 细胞因子、病毒蛋白和所需的特定部位 抗终止在噬菌体lambda系统中被部分理解，并且 噬菌体HKO22几乎完全未知。其中的差异和 这两个噬菌体之间的相似性以及相互作用 他们的监管制度之间的关系正在探索中。方法是 包括遗传和生化的，并涉及突变的分离， 调节蛋白的过量生产和纯化，以及 突变地点的建设。 另一种噬菌体调控系统，抑制，受制于 主机函数，RPSB(S2)。对照在mrna水平上。 翻译；这一规定的含义将由 突变体分析及体外翻译系统的建立。

The study of the ten"rate bacteriophage has been exceedingly useful in generating information about universal regulatory mechanisms. Regulation of gene expression by a system of transcription termination - antitermination, first described in phage, has now been seen in eukaryotic cells, in particular the control of c-myc and HIV gene expression. The cellular factors, viral proteins, and specific sites required for antitermination are partially understood in the phage lambda system, and almost completely unknown for phage HKO22. The differences and similarities between these two phages, as well as the interactions betwen their regulatory systems, is being explored. The approach is both genetic and biochemical, and involves the isolation of mutants, the overproduction and purification of regulatory proteins, and the construction of mutant sites. Another phage regulatory system, repression, is subject to control by a host function, RpsB (S2). The control is at the level of mRNA translation; the mehanism of this regulation will be determined by analysis of mutants and establishment of an in vitro translation system.