ROLE OF INTEGRATIVE HOST FACTOR IN GENE EXPRESSION

大肠杆菌的组蛋白样蛋白IHF直接作用于允许 Lambda基因的翻译，CII。我们的假设是它实现了 这是通过改变CII文字稿的结构，使其成为 核糖体可接近的；IHF结合部位与CII相邻 Share-Dalgarno序列。我们希望通过以下方式来分析这一监管体系 遗传和生化方法。前者是由我们的 构建大量的CII基因和操纵子融合；后者由我们的 建立反映翻译的转录-翻译耦合系统 IHF对CII表达的要求。我们还计划调查 CII基因的结构。最后，我们提供了选择协议来分离 CII表达不依赖于IHF的宿主突变体；这些突变体可能 显示由IHF突变引起的其他表型的变化。

An E. coli histone-like protein, IHF, acts directly to allow the translation of a Lambda gene, cII. Our hypothesis is that it accomplishes this by altering the structure of the cII transcript so that it becomes accessible to ribosomes; an IHF binding site lies adjacent to the cII Shine-Dalgarno sequence. We wish to analyze this regulatory system by genetic and biochemical approaches. The former is facilitated by our construction of numerous cII gene and operon fusions; the latter by our establishment of coupled transcription-translation system which reflects the IHF requirement for cII expression. We plan also to probe the structure of cII mRNA. Finally, we offer selection protocols to isolate host mutants in which cII expression is IHF-independent; these mutants may display alterations in the other phenotypes caused by an IHF-mutation.