STRUCTURE OF CYTOCHROMES

总体目标是确定LOW的结构和动态 分子量细胞色素与其电子传递功能有关 催化剂。细胞色素是一种普遍存在的电子传递蛋白，在 所有的细胞系。对它们的功能的理解将提供 将基础知识转化为能量代谢的中心过程。这个 主要技术将是具有二维重音的核磁共振波谱 解决方案研究。主要实验体系为细胞色素C551。 产自铜绿假单胞菌和斯图泽里假单胞菌。具体目标 (A)以氧化和还原形式指认它们的质子光谱， (B)将它们的结构相互比较，并与更大的真核生物进行比较 细胞色素，(C)确定作为探针的酰胺NH交换的动力学 (D)评估非氨基酸的重要性 决定最终三级构象的要素。

The overall objective is to determine how the structure and dynamics of low molecular weight cytochromes relate to their function as electron transport catalysts. Cytochromes are ubiquitous electron transport proteins found in all cell lines. An understanding of their function will provide fundamental knowledge into a central process of energy metabolism. The main technique will be NMR spectroscopy with accent on two dimensional solution studies. The primary experimental systems are cytochromes c551 from Pseudomonas aeruginosa and Pseudomonas stutzeri. The specific aims are to (a) assign their proton spectra in the oxidized and reduced forms, (b) compare their structures to each other and to larger eukaryotic cytochromes, (c) determine the kinetics of amide NH exchange as a probe for internal flexibility, and, (d) assess the importance of non-amino acid elements in determining the final tertiary conformation.