PROPERTIES OF UTERINE EPITHELIUM RELATED TO IMPLANTATION

拟议研究的目的是确定和表征 子宫上皮表面的特性， 滋养层粘附和随后的植入过程中的侵入。的 研究将检验上皮细胞作为原发性 调节子宫接受性的部位， 胚泡着床该项目的目标包括：1）使用 鉴定和分离子宫和滋养层的生化方法 这些膜分子以互补的方式彼此特异性结合， 受体/配体相互作用，2）验证结构和生物化学 体外子宫上皮系统作为模型的特性 接受子宫表面，并酌情完善系统，以及3） 用上皮细胞培养检查滋养层-子宫粘连， 为了测试所鉴定的受体/配体分子的功能作用， 在目标1下。该模型，作为研究的日期，提供了承诺，允许 细胞-细胞所需母体和胚胎因子的鉴定 这些基因完全不同的组织之间的粘连和融合。结果 将适用于阐明细胞相互作用中的问题， 更具体地说，涉及建立怀孕的过程。

The objective of the proposed research is to identify and characterize properties of the epithelial surface of the uterus which are involved in trophoblast adhesion and subsequent invasion during implantation. The research will test the hypothesis that the epithelium serves as the primary site for mediation of hormonally-modulated receptiveness of the uterus to blastocyst implantation. Aims of the project include 1) the use of biochemical methods to identify and isolate uterine and trophoblast membrane molecules which bind specifically to one another in complementary receptor/ligand interactions, 2) to validate the structural and biochemical properties of an in vitro uterine epithelial system as a model for the receptive uterine surface, and to refine the system as appropriate, and 3) to use the epithelial cultures to examine trophoblast-uterine adhesion and to test the functional roles of the receptor/ligand molecules identified under Aim 1. The model, as studied to date, offers promise for allowing identification of maternal and embryonic factors required for cell-cell adhesion and fusion between these genetically disparate tissues. Results will be applicable to elucidating problems in cell-interaction generally and, more specifically, to the process of establishing pregnancy.