MAINTENANCE AND REPAIR OF VASCULAR ENDOTHELIUM

我们的具体目标是：1）进一步表征脑和垂体 已经通过FPLC纯化的成纤维细胞生长因子（FGF），通过测定 其主要结构。 脑与垂体成纤维细胞生长因子的关系 也将使用针对这两种抗体开发的单克隆抗体来确定 分子。 与内皮细胞生长因子的关系 （ECGF）将被确定。 2)牛脑与 垂体FGF与培养的内皮细胞， 血管领土和物种将被确定和FGF受体 表征了 3)成纤维细胞生长因子对内皮细胞的影响 迁移，以及其作为体外趋化剂的可能作用 将被确定。 4)最后，使用抗FGF的单克隆抗体， 其在已知的各种组织、肿瘤或培养细胞中的分布 含有血管生成因子在体内将是eq.测定 血管生成 FGF在三种不同的体内模型（兔角膜、角膜基质、角膜基质）中的活性 囊和鸡绒毛尿囊膜）以及 FGF抗体在体内阻断这种活性的能力。 中的系数 将测定模拟FGF作用的基底膜（BM）。 成纤维细胞生长因子对这些因子在细胞中合成和整合的影响 将分析由培养的血管内皮细胞合成的BM。 BM和FGF两者对细胞的维持和周转的影响是通过观察细胞的生长和增殖来确定的。 内皮细胞分裂所需的转铁蛋白受体，和 其在体内（红细胞生成系统）或在器官培养（后肾 间充质），已被证明是诱导和相关的 将分析特定BM组分的外观。

Our specific aims are: 1) To further characterize brain and pituitary fibroblast growth factors (FGF) already purified by FPLC, by determining their primary structure. The relationship between brain and pituitary FGF will also be determined using monoclonal antibodies developed against both molecules. Their relationship with the endothelial cell growth factor (ECGF) will be determined. 2) The interaction of bovine brain and pituitary FGF with cultured endothelial cells originating from various vascular territories and species will be determined and the FGF receptors characterized. 3) The effect in vitro of FGF on endothelial cell migration, as well as its possible role as a chemotactic agent in vitro will be determined. 4) Finally, using monoclonal antibodies agaist FGF, its distribution in various tissues, neoplasms, or cultured cells known to contain angiogenic factors in vivo will be eq. determined. The angiogenic activity of FGF in three different in vivo models (rabbit cornea, Hamster pouch, and chick chorioallantoid membrane) will be analyzed as well as the ability of FGF antibodies to block such activity in vivo. The factor(s) in basement membranes (BM) which mimics the effect of FGF will be determined. The effect of FGF on the synthesis and integration of such factors in the BM synthesized by cultured vascular endothelial cells will be analyzed. The effect of both BM and FGF on the maintenance and turnover of the transferrin receptor which is required for endothelial cells to divide, and which in vivo (erythropoietic system), or in organ culture (metanephric mesenchyme), has been shown to be inducible and to correlate with the appearance of specific BM components will be analyzed.