HEMOGLOBIN BASED OXYGEN CARRIERS

我们建议探索人类和牛血红蛋白作为 用于输血的氧载体。 其目的是获得高 两种血红蛋白与氧的纯化的不可解离的四聚体 与体内氧运输相容的亲和力。 这项研究将 涉及必要化合物的开发、对其的全面分析 物理化学和功能特性，并对其进行初步测试 在小动物中的储存稳定性和保留时间。 一步化学 反应将用于获得所需的血红蛋白衍生物， 使用有限和特定的化学修饰，以避免形成 具有高抗原活性的产品。 已经获得的两种化合物 通过将牛血红蛋白与双-二溴水杨酰-富马酸酯交联， 广泛纯化并分析其功能特性和位点 交叉链接。 新的治疗方法将被设计用于人类血红蛋白， 交联产物的氧亲合性高于 跨功能液体

We propose to explore the potential of human and bovine hemoglobins as oxygen carriers for transfusional fluids. The aim is to obtain highly purified indissociable tetramers of the two hemoglobins with an oxygen affinity compatible with oxygen transport in vivo. The research will involve the development of the necessary compounds, full analyses of their physico-chemical and functional properties, and initial testing of their storage stability and retention time in small animals. One-step chemical reactions will be used for obtaining the desired hemoglobin derivatives, using limited and specific chemical modifications, so as to avoid formation of products with high antigenic activity. Two compounds already obtained by cross-linking bovine hemoglobin with bis-dibromosalicyl-fumarate will be extensively purified and analyzed for their functional properties and sites of cross-links. New treatments will be designed for human hemoglobin whose cross-linked products have an oxygen affinity higher than that desirable in transfunctional fluids.