PARASAGITTAL ORGANIZATION OF THE CEREBELLAR CORTEX

许多证据表明，小脑皮质有一种 矢状面旁组织。这些证据包括解剖学上的 追踪研究，电生理单一单位研究， 组织化学和免疫组织化学分析 小脑。即使这些不同的方法论已经 成功演示了一种形态的矢状面旁组织 或者是另一种，比较的尝试相对较少， 整理并合成来自这些不同数据的数据 方法论。在以下情况下，解决这个问题是绝对必要的 我们将永远确定小脑的真实性质 矢状面旁组织及其功能意义。我们有 一直在使用解剖学追踪技术研究矢状面旁 大鼠和小鼠小脑的组织。通过使用 以这些数据为起点，我们建议比较和 相比之下，在详细和彻底的方式下，旁矢状面 在不同情况下产生的组织方案 方法论方法也适用于同一物种。这 代表着我们工作的一个合乎逻辑和重要的延伸 在小脑的传入组织上做了些什么。冲向 为此，我们计划开展以下实验。(1) 在同一动物中，比较和分析 浦肯野细胞免疫组织化学染色图谱的获得 与单抗Q113和Q155结合，形成 攀缘纤维传入小脑的实验研究 WGA-HRP顺行追踪技术。(二)开展 小脑的一个小叶(小叶VIII和小叶 金字塔)的爬升方式 WGA-HRP逆行研究纤维和苔藓纤维传入 示踪技术与免疫组织化学染色模式 用单抗Q113对这个小叶进行标记。(3)进行类似的比较 5‘-核苷酸酶组织化学染色图谱分析及 传入输入的模式。(4)在下列情况下，确定受抚养人 这些染色模式中的任何一种(5‘核苷酸酶、单抗Q113和/或 Q155)关于橄榄小脑投射的完整性 单侧下橄榄复合体的化学损害。

Many lines of evidence point to the cerebellar cortex as having a parasagittal organization. This evidence includes anatomical tracing studies, electrophysiological single unit studies, histochemical and immunohistochemical analyses of the cerebellum. Even though these various methodologies have been successful in demonstrating parasagittal organization of one form or another, there have been relatively few attempts to compare, collate, and synthesize the data from these different methodologies. It is absolutely essential to attack this problem if we are ever to determine the true nature of cerebellar parasagittal organization and its functional significance. We have been using anatomical tracing techniques to study the parasagittal organization of the cerebellum in the rat and mouse. By using these data as a starting point we propose to compare and contrast, in a detailed and thorough way the parasagittal organizational schemes which result when different methodological approaches are applied to the same species. This represents a logical and important extension of the work we have been doing on afferent organization to the cerebellum. Toward this end we plan to carry out the following experiments. (1) Compare and analyze, in the same animal, the immunohistochemical staining pattern of Purkinje cells obtained with the monoclonal antibody Q113 and Q155, to the pattern of climbing fiber afferents to the cerebellum as demonstrated with WGA-HRP anterograde tracing techniques. (2) Carry out a detailed analysis of one lobule of the cerebellum (lobule VIII and the copula pyramidis) with regard to the pattern of its climbing fiber and mossy fiber inputs as studied with WGA-HRP retrograde tracing techniques and the immunohistochemical staining pattern of this lobule with mab Q113. (3) Conduct a similar comparative analysis with 5' nucleotidase histochemical staining patterns and the pattern of afferent input. (4) Determine the dependency, if any, of these staining patterns (5' nucleotidase, mab Q113, and/or Q155) on the integrity of the olivocerebellar projection by making unilateral chemical lesions of the inferior olivary complex.