PARASAGITTAL ORGANIZATION OF THE CEREBELLAR CORTEX

许多证据都表明小脑皮层具有 准共生组织 这些证据包括解剖学 追踪研究，电生理单个单位研究， 组织化学和免疫组织化学分析 小脑 尽管这些不同的方法已经 成功地证明了一种形式的准共生组织 或者其他的，比较的尝试相对较少， 整理和综合这些不同的数据， 方法论。 解决这个问题是绝对必要的， 我们要确定小脑的真正性质 及其功能意义。 我们有 一直在使用解剖追踪技术来研究 大鼠和小鼠小脑的组织。 通过使用 这些数据作为一个起点，我们建议比较， 对比，在一个详细和彻底的方式， 组织方案，当不同的 方法学方法适用于同一物种。 这 代表了我们现有工作的一个合理而重要的延伸， 对小脑传入组织的研究 朝向 为此，我们计划进行以下实验。 （一） 比较和分析，在同一种动物中， 获得的浦肯野细胞的免疫组织化学染色模式 用单克隆抗体Q113和Q155， 小脑的爬行纤维传入，如 WGA-HRP顺行追踪技术。 (2)进行 详细分析了小脑的一个小叶（小叶VIII和 金字塔接合部）关于其攀爬模式 纤维和苔藓纤维输入的WGA-HRP逆行研究 示踪技术和免疫组化染色模式 用mAb Q113处理。 (3)进行类似比较 用5'核苷酸酶组织化学染色模式分析， 传入输入的模式 (4)确定依赖关系，如果 任何这些染色模式（5'核苷酸酶，mAb Q113，和/或 问题155）橄榄小脑投射的完整性， 下橄榄复合体的单侧化学损伤。

Many lines of evidence point to the cerebellar cortex as having a parasagittal organization. This evidence includes anatomical tracing studies, electrophysiological single unit studies, histochemical and immunohistochemical analyses of the cerebellum. Even though these various methodologies have been successful in demonstrating parasagittal organization of one form or another, there have been relatively few attempts to compare, collate, and synthesize the data from these different methodologies. It is absolutely essential to attack this problem if we are ever to determine the true nature of cerebellar parasagittal organization and its functional significance. We have been using anatomical tracing techniques to study the parasagittal organization of the cerebellum in the rat and mouse. By using these data as a starting point we propose to compare and contrast, in a detailed and thorough way the parasagittal organizational schemes which result when different methodological approaches are applied to the same species. This represents a logical and important extension of the work we have been doing on afferent organization to the cerebellum. Toward this end we plan to carry out the following experiments. (1) Compare and analyze, in the same animal, the immunohistochemical staining pattern of Purkinje cells obtained with the monoclonal antibody Q113 and Q155, to the pattern of climbing fiber afferents to the cerebellum as demonstrated with WGA-HRP anterograde tracing techniques. (2) Carry out a detailed analysis of one lobule of the cerebellum (lobule VIII and the copula pyramidis) with regard to the pattern of its climbing fiber and mossy fiber inputs as studied with WGA-HRP retrograde tracing techniques and the immunohistochemical staining pattern of this lobule with mab Q113. (3) Conduct a similar comparative analysis with 5' nucleotidase histochemical staining patterns and the pattern of afferent input. (4) Determine the dependency, if any, of these staining patterns (5' nucleotidase, mab Q113, and/or Q155) on the integrity of the olivocerebellar projection by making unilateral chemical lesions of the inferior olivary complex.