DOPAMINERGIC NEURONS: CELL INTERACTIONS AND PLASTICITY

特定神经元寿命的改变可能导致严重的 成熟有机体的行为缺陷。 这里提出的调查 将研究位置和细胞的变化是否 相互作用将影响多巴胺能神经元的存活。 免疫细胞化学和组织化学方法用于鉴定 多巴胺能和假定的胆碱能神经元。 实验扰动 的多巴胺能神经元系统将进行新生儿病变 和移植技术。 发育中的中脑多巴胺能 神经元将在原位部分地去传入或移植到各种不同的组织中。 新生大鼠的大脑区域。 最初，这些研究将测试 可变环境条件的影响（例如，不同的宿主大脑 区域，传入纤维类型的差异）对连接 多巴胺能神经元的可塑性及其对这些神经元寿命的影响 神经元 定性和定量分析将在 光学和电子显微镜水平。 按照上述程序， 多巴胺能神经元和假定神经元之间细胞相互作用 将在共移植物中研究胆碱能细胞（纹状体、顶盖）， 再聚集移植物。 由于突触是一种形态实体， 表示神经元相互作用，特别强调观察 在精细结构层面。

Alterations in longevity of specific neurons may bring about severe behavioral deficits of a mature organism. The investigations proposed here will study the question of whether changes in position and cellular interactions will affect survival of dopaminergic neurons. Immunocytochemical and histochemical approaches are to be used to identify dopaminergic and putative cholinergic neurons. Experimental perturbation of the dopaminergic neuron systems will be carried out by neonatal lesions and transplantation techniques. Developing mesencephalic dopaminergic neurons will be partially deafferented in situ or transplanted to various regions of the brain of newborn rats. Initally these studies will test the influence of variable environmental conditions (e.g. different host brain areas, differences in the types of afferent fibers) on the connection plasticity of dopaminergic neurons and their effect on longevity of such neurons. Qualitative and quantitative analysis will be carried out at light and electron microscopic levels. Following the above procedures, the effect of cell interactins between dopaminergic neurons and putative cholinergic cells (striatal, tectal) will be investigated in co-grafts and reaggregation grafts. Since synapses are the morphological entities that signify neuron interactions, particular emphasis is placed on observations at the fine structural level.