An electronic-based ELISA combined with microfluidics

我们的目标是开发一种低成本的实时蛋白质检测设备，在体外培养过程中连续监测细胞因子，最终可以在临床实践中推广。我们将利用离散电子元件作为化学传感器，与独特的微流控芯片兼容，以最大限度地降低设备的总体成本，并结合小尺寸酶联免疫吸附测定（ELISA）室，最大限度地减少抗体需求。我们希望通过采用目前用于制造印刷电路板（pcb）的成熟制造技术来实现这一目标，这些技术可以毫不费力地提供定制的功能化电极，并结合um尺度的流体通道/腔室。

Our aim is to develop a low-cost real-time protein detection device to continuously monitor cytokines during in-vitro culture that could eventually expand in clinical practice. We will do this by exploiting discrete electronic components as chemical sensors that are compatible with unique microfluidic chips for minimising the overall cost of the device in combination with small size enzyme-linked immunosorbent assay (ELISA) chambers that minimise antibody requirements. We aspire achieving this by adapting well established manufacturing techniques, currently employed in fabricating printed-circuit boards (PCBs), that could effortlessly render bespoke functionalised electrodes coupled with um-scale fluidic channels/chambers.