LEUKOCYTE DEFECTS ASSOCIATED WITH THERMAL INJURY

对烫伤后白细胞的研究已经确定 吞噬细胞功能出现明显缺陷。这 观察可以用两种可能性中的一种来解释： 热损伤，白细胞生长和分化发生在 这样一种内在缺陷发展的方式防止了一些至关重要的 机械装置停止运行。或者，与 继发性烫伤对白细胞功能的影响 举止。本提案旨在评估这两种可能性。 通过利用流式细胞术技术，将允许进入- 深度分析采用烫伤大鼠烫伤模型。 流式细胞术将允许研究多个白细胞 对微升(100微升)的全血起作用。因此， 对单只大鼠的动力学研究现在是可行的。而当 在研究这种热损伤模型时，一些新的 将应用流式细胞术的应用，即使用 细胞内二氯荧光素过氧化氢测定法的评价 膜流动性作为早期发现缺陷的手段 激活事件和荧光方法的使用 同时测量吞噬功能和杀伤力。因为. 流式细胞术利用人外周血白细胞制剂的能力 混合人群，这些调查将在两个人身上进行 单核细胞和中性粒细胞吞噬细胞。开发后 和应用所需的技术，并在烧毁后 大鼠模型被更好地理解，血液来自热损伤 病人将被使用。这些病人将来自一种特殊的烧伤 该机构的单位非常热衷于协助 合作学习。这些将在#年成为重要的研究。 理解免疫应答与免疫应答之间的关系 和烫伤后的脓毒症。

Studies of leukocytes after thermal injury have determined that significant defects occur in phagocytic cell function. This observation is explained by one of two possibilities: That after thermal injury, leukocyte growth and differentiation occurs in such a way that intrinsic defects develop preventing some vital mechanism from operating. Alternatively, factors related to the thermal injury affect the leukocyte function in a secondary manner. This proposal aims at evaluating both these possibilities by utilizing flow cytometric techniques which will allow an in- depth analysis using a model of thermal injury, the burned rat. Flow cytometry will allow investigation of multiple leukocyte functions on microliter quantities (100 ul) of whole blood. Thus, kinetic studies on single rats are now practical. While investigating this model of thermal injury, a number of new applications of flow cytometry will be applied, namely the use of the dichlorofluorescein intracellular H2O2 assay, evaluation of membrane fluidity as a means of detecting defects in early activation events and the use of a fluorescence method for simultaneously measuring phagocytosis and killing. Because of the ability of flow cytometry to utilize leukocyte preparations of mixed populations, these investigations will be performed on both the monocyte and neutrophil phagocytic cells. After development and application of the required techniques, and after the burned rat model is better understood, blood from thermally injured patients will be used. These patients will be from a special burn unit at this institution which is very keen to assist in a cooperative study. These will become significant studies in understanding the relationship between immune responsiveness and sepsis after thermal injury.