MOLECULAR GENETICS OF STRESS-RESPONSES IN ARABIDOPSIS

了解有机体对压力的反应是一个基本的 生物学中的一个问题。压力反应的统一主题是 环境的扰动导致了快速而具体的 基因表达的激活。在进化过程中，植物 已经开发出有效的机制来抵抗一种 压力。基因表达的特异性诱导发生在 对环境压力的反应，如感染和 伤害；植物的“免疫反应”。这样做的一个主要目标是 一项建议是理解一种 植物会对这些胁迫做出反应。分子和遗传学 将结合各种方法来剖析这些机制 调控应激调节基因的诱导和表达 在模型植物系统中。双子叶植物拟南芥 Taliana非常适合这类研究；它的主要优势是 它可以接受常见的分子遗传操作 酵母和果蝇，但不适用于其他高等植物。 这个项目的长期目标是确定分子 与压力的信号传递和识别有关的事件。行动 应激诱导的荷尔蒙在 了解这些过程。本研究旨在分离、分离和鉴定 确定影响生物合成的基因突变的特征， 对应激诱导的乙烯和 寡糖素，以及定义应激中的DNA序列- 诱导植物防御反应基因，这些基因对 这些植物应激激素的调节。具体来说就是这个 项目目标：1)鉴定和鉴定拟南芥突变体 在应激反应中受影响的外源和 内源性低聚糖片段；2)鉴定和“应激” 乙烯“；3)分析DNA控制元件 调节乙烯和寡糖素诱导的基因；4)使用 用于识别基因/蛋白质的遗传和分子技术 直接或间接地介导乙烯的诱导-和 寡糖素调控的基因和5)构建和 表征受乙烯和乙烯调节的基因的“突变” 低聚糖素反义和正义RNA的表达 这些基因。

Understanding an organism's response to stress is a fundamental problem in biology. The unifying theme in the stress response is that perturbation of the environment leads to rapid and specific activation of gene expression. In the course of evolution, plants have developed effective mechanisms for resistance to a variety of stresses. Specific induction in gene expression occurs in response to environmental stresses such as infection and wounding; the "immune response" of plants. A major goal of this proposal is to understand the molecular and cellular basis of a plants response to these stresses. Molecular and genetic approaches will be combined to dissect the mechanisms controlling the induction and expression of stress-regulated genes in a model plant system. The dicotyledonous plant Arabidopsis thaliana is ideally suited for such studies; the major advantage is that it is amenable to molecular genetic manipulations common to yeast and Drosophlia but impractical for other higher plants. The long term goal of this project is to ascertain the molecular events involved in signalling and recognition of stress. The action of stress-induced hormones is of fundamental importance in understanding these processes. This research aims to isolate and characterize mutations in genes that affect the biosynthesis, recognition and response to stress-induced ethylene and oligosaccharins and also to define the DNA sequences in stress- induced plant defense response genes which are essential for regulation by these plant stress hormones. Specifically this project aims: 1) to identify and characterize Arabidopsis mutants that are affected in the stress response to exogenous and endogenous oligosaccharide fragments; 2) to identify and "stress ethylene"; 3) to analyze the DNA controlling elements that regulate ethylene- and oligosaccharin-induced genes; 4) to use both genetic and molecular techniques to identify genes/proteins that directly or indirectly mediate the induction of ethylene- and oligosaccharin-regulated genes and 5) to construct and characterize "mutations" in genes regulated by ethylene and oligosaccharins by expression of antisense and sense RNAs for these genes.