CAMERA SYSTEM FOR TWO-DIMENSIONAL ELECTROPHORESIS GELS

虽然已经描述了各种扫描仪和摄像系统 获取用于显示的二维电泳凝胶图案， 分析、相互比较和复制，没有一个能解决所有 一种令人满意的图像采集和识别问题 举止。这项提议是为了发展70毫米的精度。 一种用于拍摄二维图像的PIN配准相机系统 电泳图。第二光路在负极中包括a CRT生成的标识和数据显示区域。独特的设计 将镜头光斑降至最低。使用三色滤色器系统，色彩 也可以按顺序制作分色底片以用于后续的颜色 繁殖。相机系统也是一台70毫米的打印机，可以曝光 从正片到负片。可以查看正面信息，并 在合并比较器上相互比较，或为计算机化的图像进行数字化 分析。集成到打印系统中的是一种独特的技术，用于 去除可能存在于银色凝胶中的稠密背景区域。 该系统灵活，并保留原始图像以供学习或 繁殖。一台集中式数字化扫描仪可以服务于多个远程 70毫米凝胶相机。我们相信这个系统将会进入 用途最广。

While a variety of scanners and camera systems have been described for acquiring two-dimensional electrophoretic gel patterns for display, analysis, intercomparison, and reproduction, none solves all of the problems of image acquisition and image identification in a satisfactory manner. This proposal is for the development of a 70-mm precision pin-registered camera system for photographing two-dimensional electrophoretic patterns. A second optical path includes in the negative a CRT-generated identification and data display area. A unique design reduces lens flare to a minimum. Using a three color filter system, color separation negatives may also be made in sequence for subsequent color reproduction. The camera system is also a 70-mm printer which exposes positive film to the negative film. The positives may be viewed and intercompared on a merging comparator, or digitized for computerized image analysis. Incorporated into the printing system is a unique technique for removing dense background areas such as may exist in silver stained gels. The system is flexible, and retains the original image for study or reproduction. One centralized digitizing scanner can serve numerous remote 70 mm gel cameras. We believe this system is the one which will come into widest use.