COLORIMETRIC MEASUREMENT OF MILK PROGESTERONE

一种新的固相比色分析的可行性将是 调查过了。单抗将是共价的 固定在玻璃上作为固体基质。几种化学品 将对反应进行研究，以确定给予低收入的联系 蛋白质和小分子的非特异性结合 维持免疫球蛋白的完整性。玻璃将会是 经过化学精炼，以获得均匀的、可重复的涂层 与玻璃和配料的物理历史无关的抗体 到批量变型。一种新的结合物，半抗原生物素，将是 链霉亲和素-酶作为比色法示踪剂的评价 量化。得到的固相化验结果将是 最初用于测量牛乳中的黄体酮 奶牛。我们的目的是开发一种简单、可靠的检测方法 监测发情周期、产后卵巢周期、卵巢 奶牛的功能障碍和怀孕。实用的程序 要在室外测量牛奶中的孕酮浓度 实验室环境对牛也有很大的经济价值。 繁衍后代。该项目的第一阶段将确定 这样的方法，而第二阶段将专门用于 一种可在野外进行的检测方法的研制 由未受过训练的人提供的条件。

The feasibility of a novel, solid phase, colorimetric assay will be investigated. Monoclonal antibodies will be covalently immobilized on glass as the solid matrix. Several chemical reactions will be studied to identify linkages that give low nonspecific binding of proteins and small molecules while maintaining the integrity of immunoglobulins. Glass will be chemically refined to obtain a uniform, reproducible coat of antibodies independent of the physical history of glass and batch to batch variation. A new conjugate, hapten-biotin, will be evaluated with streptavidin-enzyme as a tracer for colorimetric quantification. The resulting solid phase assay will be implemented initially for measurement of progesterone in milk of cows. It is our intention to develop a simple, relaible assay for monitoring estrous cycle, ovarian cyclicity post partum, ovarian dysfunction, and pregnanacy in dairy cows. A practical procedure to measure progesterone concentration in milk outside a laboratory setting also has substantial economic value in cattle breeding. Phase I of this project will establish the feasibility of such a method while phase II will be devoted to subsequent development of an assay which can be performed under field conditions by a nontrained person.