DRUGS OF ABUSE IN SALIVA

这项研究项目旨在调查使用 唾液作为定量测量的替代生物流体 滥用药物。标本采集是基于一种专利设备 在几分钟内收集超滤的唾液。AS 作为一种非侵入性的方法，唾液可以在现场不显眼地获取，而不需要 侵犯隐私和样本被篡改的风险。一旦可行性是 事实证明，这种唾液收集设备可以取代有争议的 收集尿液，并允许采样以进行后续筛查(例如 对车辆、军事、敏感工作的司机进行路边测试)。这个 使用唾液采集器进行检测的最多的 经常滥用的药物，可卡因及其主要代谢物， 苯甲酰ecGonine，将作为第一阶段的模型系统进行研究。 不受损害地将药物从唾液转移到设备中，潜在的非 药物的特异性摄取和稳定性将被研究。最后， 尿液、血液和唾液中可卡因和苯甲酰ecGonine的浓度 来自参加戒毒计划的患者将被关联起来。 在二期期间，采集装置的应用将得到扩展 其他滥用药物并与比色法有关的应用 可供未经培训的人员现场使用的免疫分析系统 在非实验室条件下。

This research project is designed to investigate the feasibility to use saliva as an alternative biological fluid for the quantitative measurement of drugs of abuse. Collection of specimen is based on a patented device for the collection of an ultrafiltrate of saliva within a few minutes. As a non-invasive method, saliva can be unobtrusively obtained on-site without invasion of privacy and the risk of sample tampering. Once feasibility is proven, the saliva collection device could substitute for the controversial collection of urine, and allow sampling for subsequent screening (e.g. roadside testing of drivers of vehicles, military, sensitive jobs). The use of saliva collection device for the detection of one of the most frequently abused drugs, cocaine, and its major metabolites, benzoylecgonine, will be investigated as a model system in Phase I. The unimpaired transfer of drugs from saliva into the device, potential non- specific uptake, and the stability of drugs will be studied. Finally, the concentrations of cocaine and benzoylecgonine in urine, blood, and saliva from patients attending a drug rehabilitation program will be correlated. During Phase II, the application of the collection device will be extended to other drugs of abuse and applied in connection with a colorimetric immunoassay system that can be utilized on-site by untrained personnel and under non-laboratory conditions.