FIBEROPTIC REFLECTANCE/PHOSPHORESCENCE DECAY SPECTROMETE

描述（改编自申请人摘要）：本研究项目 涉及光学方法的发展，以量化总的可用 心外膜组织的氧含量。 三个病人的心外膜氧含量 主要池-溶解在水介质中的氧， 血红蛋白和氧与肌红蛋白结合。 在这一提议中， 将开发仪器，以同时获得反射率 来自心外膜的同一区域和来自 该数据确定心脏循环中的PO 2，并获得 氧合血红素蛋白和脱氧血红素蛋白。该反射率的预测能力 方法将在散射中用血红蛋白/肌红蛋白混合物进行测试 培养基和血液灌注的大鼠心脏中。 在第一阶段论证可行性后，第二阶段将集中于 开发用于灌注心脏工作和核磁共振的仪器 磁铁，以增加其效用的心脏生物能量的研究。 应用多元统计技术分析光学 光谱开辟了监测多种代谢参数的前景 同时用于评估心脏缺血和缺氧。这 仪器将量化组织代谢和氧气多个方面 研究和临床目的的供应/需求。

DESCRIPTION (Adapted from applicant's abstract):This research project involves the development of optical methods to quantify the total available oxygen content of epicardial tissue. Epicardial oxygen is found in three major pools - as oxygen dissolved in aqueous media, as oxygen bound to hemoblobin and as oxygen bound to myoglobin. In this proposal, instrumentation will be developed to simultaneously obtain reflectance spectra and phosphorescence from the same region of the epicardium and from this data determine P02 in cardiac circulation and obtain concentrations of the oxy and deoxy hemeproteins. Predictive ability of this reflectance method will be tested with hemoglobin/myoglobin mixtures in scattering media and in blood perfused rat hearts. After demonstration of feasibility in Phase I, Phase II will concentrate on developing the instrument for use in working perfused hearts and in NMR magnets to increase its utility for cardiac bioenergetic studies.The application of multivariate statistical techniques to analyze optical spectra opens up the prospect of monitoring multiple metabolic parameters simultaneously for use in evaluating cardiac ischemia and hypoxia. This instrument will quantify multiple aspects of tissue metabolism and oxygen supply/demand for research and clinical purposes.