REGIONAL FLOW IMAGING BY FLUORESCENT MICROSPHERES

这项研究的长期目标是开发一种新的方法， 微区域环流评价 本研究以 荧光微球可以在空间上定位的工作假设 通过器官连续切片的荧光成像。该项目将开发 仪器和程序，以评估整个区域流动 器官. 在第一阶段，将使用计算机控制的成像系统， 荧光微球的组装及其在灌流大鼠体内的成像 心脏会被拿走。将制定数据分析程序， 确定微区域流动特征第一阶段研究将 论证工作假设的可行性并提供设计 在第二阶段建造的成像系统的参数 更大的心脏。 该项目将为研究人员提供 快速自动评估微区域流量的仪器 在组织中使用与放射性微球相同的程序 流量测定

The long term goal of this research is to develop a new approach to the evaluation of microregional circulation. This research is based on the working hypothesis that fluorescent microspheres can be spatially located by fluorescence imaging of organ serial sections. The project will develop instrumentation and procedures to evaluate regional flow in an entire organ. During Phase I a computer-controlled imaging system will be assembled and images of fluorescent microspheres embedded in perfused rat hearts will be taken. Data analysis procedures will be developed to determine microregional flow characteristics Phase I studies will demonstrate the feasibility of the working hypothesis and provide design parameters for imaging systems to be constructed during Phase II for use with larger hearts. This project will provide researchers with the instrumentation to rapidly and automatically evaluate microregional flow in tissue using the same procedures involved in radioactive microsphere flow determinations.