NUCLEIC ACIDS RAMAN OPTICAL ACTIVITY

这个项目的目标是开发一种能够 阐明复杂核酸的结构信息。理想情况下， 任何用于核酸分析的技术不仅应该产生 将揭示核苷酸序列的结构细节，但将 还为终端用户提供三维构象 信息。例如，分析这三个变量的能力 DNA双螺旋的空间结构可以导致 了解遗传性状转移的细节。我们 建议通过探测来阐明结构和构象信息 通过快速成熟的核酸中的手性中心 拉曼光学活性(ROA)光谱技术。高效 传统拉曼光谱和ROA光谱的利用 将使最终用户能够确定结构和构象 来自核酸样本的信息。 第一阶段的目标是确定快速结构的可行性 核苷和核苷酸的构象鉴定， 具有ROA光谱学的核酸构建块。在阶段中 我计划我们将尝试建立敏感度水平 ROA将能够快速检测核酸，并将收集 来自核酸的代表性光谱。在第二阶段计划期间 还将对几种核酸进行更详细的分析。 作为对小(2-4个核苷酸单位)的详细光谱研究 寡核苷酸，以便将某些ROA光谱特征与 特定的核苷酸序列和构象。在第二阶段期间 编写了一个ROA采样附件的原型，可以在 任何商业拉曼系统，都将被开发出来。

The objective of this program is to develop a technique that can elucidate structural information from complex nucleic acids. Ideally, any technique employed for nucleic acid analysis should yield not only structural details which will reveal the nucleotide sequence, but will also provide the end user with three dimensional conformational information. For example, the ability to analyze variations in the three dimensional structure of the DNA double helix can lead to an improved understanding of the details of the transfer of genetic traits. We propose to elucidate structural and conformational information by probing the chiral centers within the nucleic acids via the rapidly maturing spectroscopic technique of Raman Optical Activity (ROA). Efficient utilization of the traditional Raman spectrum as well as the ROA spectrum will enable the end user to determine structural and conformational information from nucleic acid samples. The Phase I objective is to determine the feasibility of rapid structural and conformational identification of nucleosides and nucleotides, the nucleic acid building blocks, with ROA spectroscopy. During the Phase I program we will attempt to establish the sensitivity levels at which ROA will be capable of rapid detection of nucleic acids and will collect representative spectra from a nucleic acid. During the Phase II program a more detailed analysis of several nucleic acids will be pursued as well as detailed spectral studies of small (2-4 nucleotide units) oligonucleotides in order to correlate certain ROA spectral features with specified nucleotide sequences and conformations. During the Phase II program a prototype ROA sampling accessory, which can be implemented into any commercial Raman system, will be developed.