13C-LABELED TRIOLEIN FOR DIAGNOSTIC BREATH TESTS

~(14)C-三油酸呼气试验是一种流行的替代经典的方法。 72小时粪便脂肪采集用于测量脂肪吸收不良 简单性和可以做出诊断的快速。然而， 因为对低水平辐射的长期影响的担忧 (尤其是当摄入时)，这种测试通常不用于儿童 或育龄妇女。放射性碳的替代 具有稳定的同位素(13C)和使用同位素比率进行检测 质谱仪，现在已经向任何人提供了三油酸呼气测试 受试者的数量。目前使用它的一个主要限制是 13C标记的三油酸甘油酯成本较高。 我们提出了一种生物合成三油酸脂的方法 将导致标签的含量增加18倍。 PERLABEL(100%掺入)13C-三油酸酯将由 一株高油酸产油菌的自养生长 以13CO2为唯一碳源。我们希望这项技术 在这笔赠款中开发的将降低生产成本并增强 这种化合物的敏感性，使每种产品的成本降低10倍 可以实现耐心测试。通过降低此测试成本并消除 摄取放射性化合物，三油酸呼气测试应该 更容易被临床医生用于妇女的诊断 还有孩子。

The 14C-triolein breath test is a popular alternative to the classical 72-hr fecal fat collection for measuring fat malabsorption because of its simplicity, and the rapidity with which a diagnosis can be made. However, because of the concern for the long term effects of low-level radiation (especially when ingested), this test is generally not used in children or women of child-bearing age. The replacement of the radioactive carbon with its stable isotope (13C) and its detection using an isotope ratio mass spectrometer, has now made the triolein breath test available to any population of subjects. A principal limitation in its use today is the high cost of 13 C-labeled triolein. We are proposing a biological approach to the synthesis of triolein which will result in an 18-fold increase in the content of the label. Perlabeled (100% incorporation) 13C-triolein will be produced by the autotrophic growth of a high-oleic oil producing strain of microalgae using 13CO2 as the sole carbon source. It is our hope that the technology developed in this grant will reduce the production costs and enhance the sensitivity of this compound such that a 10-fold reduction in cost per patient test can be realized. By reducing this test cost and eliminating the ingestion of radioactive compounds, the triolein breath test should be more readily available to the clinician for diagnostic uses in women and children.