CAMERA/SCANNER FOR GENOMIC MAPPING AND PROTEIN ANALYSIS

为了人类基因组测序计划 蛋白质索引项目，许多图像必须记录， 分析了 图像包括那些一维的 DNA测序凝胶的放射自显影，一维DNA 凝胶使用荧光染色，和2-D模式的复杂 蛋白质混合物 有一个标准化的要求， 宽频带组合式电子录音系统 动态范围，为电子图像处理提供图像 并进行目视检查、相互比较、出版， 质量控制 我们已完成可行性和设计研究 用于70 mm胶片电子扫描仪和图像分析仪的组合 具有宽动态范围、阳性样品识别和文本 内含物、胶片图像的后续电子扫描、随机 访问大型图像库，并对 模式. 使用滤镜、分色和色彩转换 可以准备底片。 标准化靶板允许胶片 标准化，以及不同方法结果的相互比较 laboratories. 一系列复杂度分级的摄像机， 计划将小型实验室纳入中央数据库， 加工设施。 提供摄影的核心问题 宽动态范围存储和高对比度打印 出版已经解决了。

For the human genome sequencing project and the companion protein index project, numerous images must be recorded and analyzed. Images include those of one-dimensional autoradiographs of DNA sequencing gels, one dimensional DNA gels using fluorescent staining, and 2-D patterns of complex protein mixtures. There is a requirement for a standardized combined photographic-electronic recording system with wide dynamic range to provide images for electronic image processing and for visual inspection, intercomparison, publication, and quality control. We have completed feasibility and design studies for a combined 70mm film-electronic scanner and image analyzer with wide dynamic range, positive sample identification and text inclusion, subsequent electronic scanning of film images, random access to large image libraries, and visual intercomparison of patterns. Using filters, color separation and color translation negatives may be prepared. Standardized target plates allow film standardization, and intercomparison of results from different laboratories. A series of cameras of graded complexity are planned to allow small laboratories to be tied into central data processing facilities. The core problem of providing photographic storage of wide dynamic range and prints of high contrast for publication has been solved.