NEW APPROACHES TO FLOW SORTING OF CHROMOSOMES

通过流动分选来纯化单个染色体通常是第一次 定义正常和异常人类基因结构的步骤。这个 聚合酶链式反应大大减少了所需的样本量 用于分离染色体DNA，使其能够开发出更简单、 更便宜的染色体分类器。使用的乐器的性能 风冷紫外(UV)和蓝氦镉(He-Cd)激光 DAP1/奥利弗霉素染色的染色体的流式细胞术来源 将对Hoechst 33258/色霉素A3进行评估，以确定是否 该系统可以解析人类的核型，也可以解决现有的商业 仪器。作为替代，几种核酸荧光染料不是 以前应用于染色体分析，并有可能在大型 现有的流分选器的数量将作为附件或 目前用于双变量流式核型分析的染色的替代品。

Purification of individual chromosomes by flow sorting is often a first step in defining the structure of normal and abnormal human genes. The polymerase chain reaction drastically reduces the amount of sample required for isolation of chromosomal DNA, making it feasible to develop simpler, less expensive chromosome sorters. The performance of an instrument using air-cooled ultraviolet (UV) and blue helium-cadmium (He-Cd) laser light sources in flow cytometry of chromosomes stained with DAPl/olivomycin or Hoechst 33258/chromomycin A3 will be evaluated to determine whether the system can resolve the human karyotype as well as can existing commercial apparatus. As an alternative, several nucleic acid fluorochromes not previously applied to chromosome analysis and potentially usable in a large number of existing flow sorters will be examined as adjuncts or alternatives to stains presently used for bivariate flow karyotyping.