AUTOMATIC PARALLEL PEPTIDE SYNTHESIZER FOR AIDS VACCINE

描述：（改编自申请人摘要）目的是 开发一种多平行肽合成仪， 产生一系列不同的肽，每一种肽的量都足以 实验性免疫动物对抗病毒，包括HIV-1， 与当前生产一个所需的时间相同或更少 肽。 所采用的并联构型涉及一系列反应 转子中的腔室，其布置成通过离心力供给试剂， 或者来自与离心快速器中所用的传送盘类似的传送盘 分析仪，或从由刀片分割的试剂流 安装在转子上。 这使得肽特异性氨基酸 衍生物加入到每个反应列中，而洗涤溶液， 加入每个合成步骤通用的去封闭剂和封端剂 在旋转过程中。 通过仔细控制试剂密度差异， 通过控制转子速度来矢量化力场，解决方案如下 使用最小体积的试剂定量改变。 线的角度 并且反应列中的表面被设计成控制流体流动， 以允许一种流体被另一种流体置换。 肽被切割 并收集在同向旋转的收集环中， 旋转 该系统的许多应用进行了说明。

DESCRIPTION: (Adapted from the applicant's abstract) The objective is to develop a multiple-parallel peptide synthesizer which can efficiently produce a series of different peptides, each in sufficient quantities for experimental immunization of animals against viruses including HIV-1, in either the same or less time than currently required to produce one peptide. The parallel configuration employed involves a series of reaction chambers in a rotor arranged to be fed reagents by centrifugal force, either from a transfer disc similar to that used in the centrifugal fast analyzer, or from a reagent stream which is segmented by blades incorporated in the rotor. This allows peptide specific amino acid derivatives to be added to each reaction train, while the wash solutions, deblocking, and capping reagents common to each synthetic step are added during rotation. By careful control of reagent density differences, and by vectoring of the force field by control of rotor speed, solutions are quantitatively changed using minimal volumes of reagents. Angles of lines and surfaces in the reaction train are designed to control fluid flow and to allow displacement of one fluid by another. The peptides are cleaved and collected in a co-rotating collection ring, and lyophilized during rotation. Numerous applications of this system are described.