AUTOMATIC PARALLEL PEPTIDE SYNTHESIZER FOR AIDS VACCINE

描述：(改编自申请人的摘要)目标是 研制了一种高效的多肽合成器 产生一系列不同的多肽，每一种都有足够的数量 动物对包括艾滋病毒-1在内的病毒的实验性免疫 与当前生产所需的时间相同或更短 多肽。所采用的平行构型涉及一系列反应 通过离心力输送试剂的转子中的腔室， 或者是从类似于离心式快滤机中使用的转移盘 分析仪，或来自用刀片分割的试剂流 安装在转子中。这使得多肽特有的氨基酸 将衍生物添加到每个反应序列中，而洗涤溶液， 添加了每个合成步骤通用的解堵试剂和封端试剂 在旋转过程中。通过仔细控制试剂浓度差异，以及通过 通过控制转子速度来矢量化力场，解决方案是 用最小的试剂体积进行定量改变。线的角度 反应序列中的表面被设计成控制流体流动和 使一种液体被另一种液体置换。这些多肽被切割 收集在共同旋转的收集环中，并在冷冻干燥过程中 旋转。描述了该系统的许多应用。

DESCRIPTION: (Adapted from the applicant's abstract) The objective is to develop a multiple-parallel peptide synthesizer which can efficiently produce a series of different peptides, each in sufficient quantities for experimental immunization of animals against viruses including HIV-1, in either the same or less time than currently required to produce one peptide. The parallel configuration employed involves a series of reaction chambers in a rotor arranged to be fed reagents by centrifugal force, either from a transfer disc similar to that used in the centrifugal fast analyzer, or from a reagent stream which is segmented by blades incorporated in the rotor. This allows peptide specific amino acid derivatives to be added to each reaction train, while the wash solutions, deblocking, and capping reagents common to each synthetic step are added during rotation. By careful control of reagent density differences, and by vectoring of the force field by control of rotor speed, solutions are quantitatively changed using minimal volumes of reagents. Angles of lines and surfaces in the reaction train are designed to control fluid flow and to allow displacement of one fluid by another. The peptides are cleaved and collected in a co-rotating collection ring, and lyophilized during rotation. Numerous applications of this system are described.