RAPID EIA FOR COTININE IN BIOLOGICAL FLUIDS

烟草产品的消费将使其所有的有害影响 我国民众的总体健康，过去和现在仍然是主要的健康问题。 尽管消费香烟的人数在过去几年里有所下降 在过去的十年里，吸烟者使用更多的香烟，青少年正在 不仅消费香烟，而且大量消费无烟 烟草。还有人担心被动吸烟对 非吸烟者，如工作场所和 这个家。由于这些问题，有相当大的努力是 由美国政府提出，以实现无烟社会的目标 这将需要特定的分析程序来监测和评估 这些努力的成功。 对于特定和敏感的问题，有多种方法可用 生物体中尼古丁及其主要代谢物可替宁的测定 体液。现有的大多数分析方法(如高效液相、气相色谱、放射免疫分析)都需要 昂贵的设备、训练有素的人员和实验室环境。 我们建议开发一种酶免疫分析(EIA)，这将有助于 检测生物体液中不同浓度的可替宁。 这种分析方法可用于监测烟草的效果。 关于儿童发育和吸烟流行病学研究的产品 在民众中的地位。该分析方法简单、快速(不到5分钟)， 无仪表，因此便于携带，可在工作场所或 医生办公室。

The consumption of tobacco products will all its deletereous effects upon the general health of our populace, was and still is major health problem. Although the number of people consuming cigarettes has decreased over the last decade, smokers are using more cigarettes and adolescents are consuming not only cigarettes, but also large quantities of smokeless tobacco. There is also concern with regard to effects of passive smoke on non-smokers such as children and adolescents both in the workplace and in the home. Because of these problems, there is a considerable effort being put forth by the U.S. Government to obtain the goal of a smoke-free society which will require specific analytical procedures to monitor and evaluate the success of such efforts. A variety of methods are available for the specific and sensitive determination of nicotine and its major metabolite, cotinine, in biological fluids. Most of the available assays (i.e., HPLC, GC, RIA) require expensive equipment, trained personnel, and a laboratory setting. We propose to develop an enzyme immunoassay (EIA) which will be useful for the detection of various concentrations of cotinine in biological fluids. This assay can be adapted for use in monitoring the effects of tobacco products on child development and for epidemiological studies on smoking status in the populace. The assay is simple, fast (less than 5 minutes), noninstrumented, and thus, easily portable for use in the workplace or physicians' office.