EKS CENTER REQUEST FOR VG MM707E MS WITH LC INTERFACE

本建议书中要求的仪器将提供独特的 NIH支持的一组调查人员的能力，他们有共同的 需要高度敏感的分析和结构表征 方法测定复合脂类和低聚糖。私家侦探和合作者 与现有的Finnigan 4500质谱仪和移动皮带液体 层析界面，已经证明了在线LC/MS 未烷化磷脂和糖脂的分析可提供快速 分析和结构信息以前是不可能的。弥撒 芬兰4500(1000 U)的射程是对 分子量以上复合脂类的LC/MS开发 1000道尔顿。人们还迫切要求使用现有的 来自正在进行的研究计划的质谱学设施。建议数 VG MM707E(以前称为7070E-HF)高质量的采购 具有DCI和LC接口的射程质谱仪(HF-MS)将提供 波士顿地区高质量在线LC/MS的独特能力 分析和带状FAB电离或源内闪蒸。这个 现有仪器将被释放，用于需要MS的研究项目 低分子物质的分析。新的仪器将 直接和唯一地支持中描述的研究计划的需求 应用，即方法开发(McCluer等)、分析和 中性鞘糖脂(Schwarting，McCluer)的结构鉴定 和Raghavan)，神经节苷脂(McCluer，Jungalwala和Schwarting)复合体 磺基糖脂在异染性脑白质营养不良(Kolodny)，磷脂 髓鞘生成与脑发育调控的相关分析 肿瘤(Jungalwala)和与甘露糖苷病相关的寡糖分析 (丹尼尔)。因此，这种具有DCI和LC接口的共享先进的HF-MS 能力将服务于需求，并促进正在进行的 NIH支持研究项目，并无疑将为 追求以前无与伦比的新研究目标。

The instrumentation requested in this proposal will provide a unique capability for a group of NIH supported investigators who have a common need for highly sensitive analytical and structural characterization methods for complex lipids and oligosaccharides. The PI and collaborators, with the existing Finnigan 4500 mass spectrometer and a moving belt liquid chromatographic interface, have demonstrated that the on-line LC/MS analysis of underivatized phospholipids and glycolipids can provide rapid analytical and structural information previously not possible. The mass range of the Finnigan 4500 (1000 u) is a severe limitation to the exploitation of LC/MS for complex lipids which have molecular weights above 1000 daltons. There are also pressing demands for access to the existing mass spectrometry facilities from ongoing research programs. The proposed acquisition of a VG MM707E (formerly referred to as 7070E-HF) high mass range mass spectrometer (HF-MS) with DCI and LC interface will provide a unique capability in the Boston area for on line LC/MS with high mass analysis and belt FAB-ionization or in-source flash evaporation. The existing instrumentation will be freed for research programs requiring MS analysis of low molecular weight materials. The new instrumentation will directly and uniquely support the needs of research programs described in the application i.e. methods development (McCluer, et al), analysis and structural elucidation of neutral glycosphingolipids (Schwarting, McCluer and Raghavan), gangliosides (McCluer, Jungalwala and Schwarting) complex sulfoglycolipids in metachromatic leucodystrophy (Kolodny), phospholipid analysis related to regulation of myelinogenesis and development of brain tumors (Jungalwala) and oligosaccharide analysis related to mannosidoses (Daniel). Thus, this shared advanced HF-MS with DCI and LC interfacing capability will serve the needs and enhance the progress of these ongoing NIH supported research programs and will undoubtedly contribute to the pursuit of new research goals previously unapproachable.