CHARACTERIZATION OF FATTY ACID METABOLITES BY GC/MS

去饱和是膳食亚油酸改性的必要步骤 和亚麻酸分别转化为花生四烯酸和二十二碳六烯酸。 这些酸的水平被肝脏和大脑中的乙醇降低， 这很可能会导致一些病理后果， 与酗酒有关。因此，我们正在研究基本的 去饱和酶系统的生物化学，并观察两者的作用 饮食和酒精暴露对酶活性的影响。这一过程的初始阶段 这项工作需要发展去饱和酶的测定方法 使用稳定的同位素标记的脂肪酸的体内和体外活性 选择离子监测电子俘获负电离酸 GC/MS。色谱，电子碰撞，和 五氟苄基的负离子电子捕获质谱 各种脂肪酸的衍生物已经开始。方法 从极性和中性脂质生产PFB衍生物，以及 随后的纯化程序和回收实验正在进行中。 发展大鼠、小鼠和豚鼠的体外和体内研究 将为人类协议的发展提供基础。我们 研究了脂质饮食调整和慢性 乙醇暴露对2-系列甘草素产生和释放的影响 从老鼠的大脑切片中提取的给大鼠喂食不同脂肪来源的食物， 两周，并通过吸入暴露于酒精再一周。 体外试验结果表明，饮食和乙醇 治疗显著影响脑切片的前列腺素释放。

Desaturation is a necessary step in the modification of dietary linoleic and linolenic acids to arachidonic and docosahexaenoic acid, respectively. The levels of these acids are lowered by ethanol in the liver and brain and it is likely that this leads to some of the pathological consequences associated with alcoholism. We are therefore examining the basic biochemistry of the desaturase system and observing the effects of both diet and alcohol exposure on enzymatic activity. The initial phase of this work requires the development of methodology for assay of desaturase activity both in vivo and in vitro using stable isotopically labeled fatty acids by selected ion monitoring electron capture negative ionization GC/MS. The characterization of the chromatographic, electron impact , and negative ion electron capture mass spectra of the pentafluorobenzyl (PFB) derivatives of the various fatty acids has been initiated. Methods for producing the PFB derivatives from polar and neutral lipids as well as subsequent purification procedures and recovery experiments are under development. In vitro and in vivo studies with rats, mice,and guinea pigs will provide a basis for the development of human protocols. We investigated the effects of both lipid dietary modifications and chronic ethanol exposure on the production and release of 2-series prostaglandins from rat brain slices. Rats were given diets with different fat sources for two weeks and exposed to alcohol via inhalation for an additional week. Results of the in vitro procedure showed that both diet and ethanol treatments significantly affected prostaglandin release from brain slices.