CORE--REMEDIATION PRODUCT EVALUATION

超级基金的生物和化学修复工艺 化学品被认为会产生毒性较小的产品。这个 核心的总体目标是测试这一假设的一些 计划项目调查员采用的补救策略。 核心的一个方面将是产生补救产品 超级基金化学品公司。这将包括但不限于， 还原脱氯制样方法的研究 多氯联苯(多氯联苯)由#年生产科产生 核心。人们对多氯联苯的担忧是因为它们在全球 分布、环境持久性和各种 毒害已被归因于它们。还原脱氯是 一种新的和重要的多氯联苯的厌氧微生物转化 已经被这个核心和其他地方的调查人员广泛研究过。 这一过程从联苯环上除去氯，导致 含有越来越少的氯化同系物的混合物。这 由于以下原因，转化可能导致生物活性的变化 所得多氯联苯混合物的组成不同，因为 个别同类者可能在作用机制(S)和 威力。厌氧脱氯产生的生物降解产物 提交给核心的多氯联苯和其他补救产品将 使用一系列可用的测试进行生物活性测试 该计划项目的生物医学研究人员实验室。这些 测试包括(例如)对细胞间影响的分析。 通讯、中性粒细胞功能、淋巴细胞转化、P-450 诱导，并激活蛋白激酶C。该组织的活动 这些检测中的补救产品将与 启动的超级基金化学品公司。由此产生的信息 测试对于修改补救方案将是有价值的，以确保 生物活性降低，没有有毒副产物。在……里面 此外，这一努力代表着 补救研究和本计划中提出的健康危害研究 计划项目，并将帮助解决超级基金关注的问题。

The processes of biological and chemical remediation of Superfund chemicals are assumed to generate products with less toxic effects. The overall objective of the Core is to test that assumption for some of the remediation strategies employed by Investigators of the Program Project. One aspect of the core will be to generate remediation products of Superfund chemicals. This will include, but not be limited to, production of samples from the reductive dechlorination of polychlorinated biphenyls (PCBs) generated by the Production Section of the Core. Concern about PCBs has arisen because of their global distribution, environmental persistence and the fact that a variety of toxic effects have been ascribed to them. Reductive dechlorination is a novel and important anaerobic microbial transformation of PCBs that has been studied extensively by investigators in this Core and elsewhere. This process removes chlorine from the biphenyl ring resulting in mixtures containing fewer and less chlorinated congeners. This transformation may result in a change in biological activity due to differences in the composition of the resultant PCB mixtures and because individual congeners probably differ in mechanism(s) of action and potency. Biodegradation products from the anaerobic dechlorination of PCBs, and other remediation products submitted to the Core, will be assayed for biological activity using a number of tests available in the laboratories of Biomedical Investigators of the Program Project. These tests include (for example) assays for effects on intercellular communication, neutrophil function, lymphocyte blastogenesis, P-450 induction, and protein kinase C activation. The activity of the remediation products in these assays will be compared to the activity of the starting Superfund chemicals. Information resulting from this testing will be valuable in modifying remediation schemes to ensure reduction of biological activity and absence of toxic byproducts. In addition, this effort represents an important link between the remediation research and the health hazards research proposed within this program project and will aid in resolving issues of Superfund concern.