CORE--BREAST CANCER CELL LINE RESOURCE

这个核心机构的主要功能是管理乳腺癌 细胞系资料库，并协助乳腺癌研究人员使用细胞 基于文化的研究。该设施提供共享的组织培养区域 它们装备精良，备有所有必要的组织 培养试剂和用品。乳腺癌细胞系被培养并 作为生长或冷冻的细胞库提供给调查人员。 冻结的细胞库存会根据需要进行补充。乳腺癌细胞 管理容量为48,000瓶细胞的生产线存储库 通过一个完整的库存系统和一个计算机数据库。一批 提供与组织培养相关的服务，包括支原体检测， 辅助病毒拯救试验，小牛血清的木炭剥离和生长 新一批胎牛血清和小牛血清的曲线分析。基因 LacZ标记乳腺癌细胞的实验研究 逆转录病毒载体或转染质粒载体，产生 乳腺癌细胞的条件培养液及其大鼠的生产 用于动物实验的乳腺癌细胞数量正在开发中 服务。 共有35名研究人员、受训人员使用共享的组织培养区 和来自9个不同乳腺癌研究实验室的技术人员。 这些研究人员通常没有组织培养空间 单独的实验室。乳腺癌细胞系资料库由以下人员使用 来自13个不同乳腺癌研究实验室的60人。 支原体检测是所有乳腺癌患者的常规要求。 实验室。辅助病毒救援化验是最近提供的一项服务 并已被3个不同的乳腺癌实验室使用。遗传 6例乳腺癌要求用LacZ标记细胞 实验室，条件培养液的生产已被5个乳房所要求 癌症实验室，并大量生产乳腺癌细胞 应8个乳腺癌实验室的要求。

The primary functions of this core facility are to manage the breast cancer cell line repository and to assist breast cancer investigators with cell culture based research. The facility provides shared tissue culture areas which are well equipped and stocked with all of the necessary tissue culture reagents and supplies. Breast cancer cell lines are cultured and made available to investigators as either growing or frozen cell stocks. Frozen cell stocks are replenished as required. the breast cancer cell line repository, which has a capacity of 48,000 vials of cells, is managed through a complete inventory system and a computer database. A number of tissue culture related services are offered, including mycoplasma testing, helper virus rescue assays, charcoal stripping of calf serum, and growth curve analysis of new lots of fetal calf serum and calf serum. The genetic labeling of breast cancer cells with lacZ by either transduction with a retroviral vector or transfection with a plasmid vector, the production of conditioned media from breast cancer cells and the production of large numbers of breast cancer cells for animal experiments are developing services. The shared tissue culture areas are utilized by 35 investigators, trainees and technicians from 9 different breast cancer research laboratories. These researchers generally do not have tissue culture space in their individual laboratories. The breast cancer cell line repository is used by 60 individuals from 13 different breast cancer research laboratories. Mycoplasma testing is regularly requested by all of the breast cancer laboratories. The helper virus rescue assay is a recently offered service and has been used by 3 different breast cancer laboratories. Genetic labeling of cells with lacZ has been requested by 6 breast cancer laboratories, conditioned media production has been required by 5 breast cancer laboratories, and bulk breast cancer cell production has been requested by 8 breast cancer laboratories.