BIOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF SIGMA RECEPTORS

这个项目描绘了生物化学、化学和药理学 σ受体和配体的性质。 检查Sigma配体 用于调节NMDA受体复合物介导的学习 流程. 学习能力的评估使用自发的 交替在Y-迷宫测试空间工作记忆，和 跳台被动回避和高架十字迷宫试验 记忆 PRE-084是本实验室发现的一种选择性σ配体， 减轻MK-801诱导的所有三种记忆过程的损害 试验. PRE-084的这些作用被σ受体拮抗 拮抗剂BMY-14802。 溶解的σ受体被部分纯化 使用阴离子交换和疏水相互作用色谱法。 在整个色谱过程中，具有强的 415 nm处的吸光度与σ受体共迁移。 结果 这表明σ受体可能与一种 发色团 受体的进一步纯化正在进行中。

This project delineates biochemical, chemical, and pharmacological properties of sigma receptors and ligands. Sigma ligands are examined for a modulation of the NMDA receptor complex-mediated learning processes. Learning capacities are evaluated using the spontaneous alternation in a Y-maze test for spatial working memory, and the step-down passive avoidance and the elevated plus-maze test for long-term memory. PRE-084, a selective sigma ligand discovered at this laboratory, attenuate MK-801-induced impairment of memory processes in all three tests. These effects of PRE-084 are antagonized by a sigma receptor antagonist BMY-14802. Solubilized sigma receptors are partially purified using anion-exchange and hydrophobic interaction chromatographies. Throughout the chromatographic procedures, a chromophore with a strong absorbance at 415 nm co-migrates with sigma receptors. The result suggests that sigma receptors may have a close relationship with a chromophore. Further purification of the receptors is underway.