RESOURCES FOR PROTEIN CRYSTALLOGRAPHY

加州大学圣迭戈分校的蛋白质结晶学资源 自七年前开业以来，取得了成功。每年我们都会收到， 平均而言，来自外部用户的好建议约有20个，其中约 已有12个小组获得了使用Mark II高速数据的时间 收集系统。在七年时间里，高强度 对100多个不同项目进行了测量。数据一直是这样的 很好，这些项目中的许多项目都在 在数据收集几个月后。 在未来五年内，我们希望扩大资源设施 这样我们才能更好地为蛋白质结晶学社区服务。第一 我们想设置Mark IV数据采集系统，可以使用 要采集小晶体的数据或用非常小的 大的单位细胞尺寸(就像高达500A的小病毒)。这个系统 将有一个非常高亮度的旋转阳极X射线发生器( Rigaku FR发电机)。我们还希望继续探索这一想法 使用金阳极建立多波长衍射仪，可以 通过在蛋白质晶体上收集TA来解决相态问题 浸泡在铂试剂中。最后，但并非最不重要的是，我们希望 探索一种产生不同波长X射线的全新方法 使用康普顿后向散射效应。

The U.C.S.D. Resource for Protein Crystallography has been extremely successful since its opening seven years ago. Each year we have received, in average, about 20 good proposals from outside users, of which about twelve groups have been given time on the Mark II high-speed data collection systems. During the seven year period, the high intensity measurements for more than 100 different projects. The data have been so good that many of these projects obtained good electron density maps within a few months after the data were collected. During the next five years we would like to expand the resource facilities so that we can better serve the protein crystallography community. First we would like to set up the Mark IV data collection system that can be used to collect data for small crystals or to collect data of crystals with very large unit cell dimensions (like small viruses up to 500 A). This system will have a rotating anode X-ray generator with very high brightness (the Rigaku FR generator). We would like also to continue to explore the idea of using the gold anode to set up a multiwavelength diffractometer that can solve the phase problem by collecting ta on a protein crystal that has been soaked in a platinum reagent. Last, but not least, we would like to explore a completely new way to generate X-rays at different wavelengths using the Compton Backscattering effect.