MOLECULAR GENETIC ANALYSIS OF LYMPHOCYTE FUNCTION

这些研究的重点是MHC的功能作用 I类分子和模型系统的发展，以检查这一点 体外功能 过去一年的具体成就包括： 1)参与蛋白水解途径的表征 不同I类限制性肽的细胞外加工; 2） HIV-1 gp 160衍生的最小肽的表征 由H-2Dd和I-Ad呈递的包膜糖蛋白; 3） 一组单链I类的生化和功能分析 工程技术产生的分子; 4）评价 MHC I类分子H-2Dd在NK细胞亚群信号传导中的作用 携带细胞表面标记物Ly-49的细胞; 5）T细胞的评估 受体/I类相互作用。

The focus of these studies has been on the functional role of the MHC class I molecule and the development of model systems for examining this function in vitro. Specific accomplishments during the past year include: 1) the characterization of proteolytic pathways involved in the extracellular processing of different class I restricted peptides; 2) the characterization of the minimal peptides derived from the HIV-1 gp 160 envelope glycoprotein that are presented by both H-2Dd and I-Ad; 3) the biochemical and functional analysis of a set of single chain class I molecules generated by engineering techniques; 4) the evaluation of the role of the MHC class I molecule H-2Dd in the signalling of a subset of NK cells bearing the cell surface marker Ly-49; 5) the evaluation of T cell receptor/class I interactions.