MODULATION OF ETS FUNCTION BY PROTEIN-PROTEIN INTERACTIONS

在人类中发现了一个不断扩大的ets相关基因家族， 基因组，以及其他物种。 此ets系列的功能 包括DNA结合结构域以及螺旋-环-螺旋基序，其 被认为是蛋白质-蛋白质相互作用的位点。 的 该项目的目标是识别与蛋白质相互作用的蛋白质。 个别ETS蛋白，从而引起功能差异 在ETS家族的成员中。 为此，定义的编码区 将人ETS 1和ETS 2基因分别克隆到原核表达载体pET 载体，其可被诱导过表达融合蛋白， 可快速纯化。 这些工程蛋白质是放射性标记的 并用于探测表达文库，以鉴定ETS 蛋白质相互作用。 初步结果显示， 似乎与ETS 1相互作用。 这种互动将进一步 研究以定义交互所需的域。 这些 方法也将被用来鉴定蛋白质， ETS家族的其他成员。

An ever-expanding family of ets-related genes has been found in the human genome, as well as in that of other species. Features of this ets family include a DNA-binding domain, as well as a helix-loop-helix motif, which has been postulated to be a site for protein-protein interaction. The goal of this project is to identify proteins which interact with individual ETS proteins, and thus give rise to functional differences among the members of the ets family. To this end, defined coding regions of the human ETS1 and ETS2 genes have been placed into prokaryotic pET vectors, which can be induced to overexpress the fusion protein, which then can be rapidly purified. These engineered proteins are radiolabelled and used to probe expression libraries to identify proteins with which ETS proteins interact. Preliminary results have revealed several clones which appear to interact with ETS1. This interaction will be further investigated to define the domains required for interaction. These methods will also be employed to identify proteins which interact with other members of the ets family.