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MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II

MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II
小鼠模型——前列腺素合成酶I和II的靶向基因敲除
批准号：
3755386
负责人：
R LANGENBACH
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3755386
关键词：
Alphaherpesvirinae alleles bacterial genetics carcinogenesis disease /disorder model drug resistance embryonic stem cell gene deletion mutation genetically modified animals hypertension laboratory mouse molecular cloning neomycin nucleic acid sequence prostaglandin endoperoxide synthase site directed mutagenesis southern blotting thymidine kinase transposon /insertion element virus genetics

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中文摘要
翻译
小鼠前列腺素合酶Ⅰ基因的部分克隆 共获得129个基因组DNA。 靶向构建体是使用 具有插入的细菌neo基因的7.5kb的基因组克隆， 尾随单纯疱疹病毒TK基因。 将构建体 电穿孔入胚胎干细胞和G418/gangcyclovir抗性 分离克隆。 同源靶向的ES细胞通过 Southern和PCR分析。 具有单个等位基因的靶向ES细胞 的PHS-1已被注射到囊胚中， 胚泡植入 嵌合体的诞生，随后， 生殖系传播动物的研究正在等待中。 这些动物将被用于 以确定PHS-1在癌发生中的作用。
英文摘要
A partial clone of the prostaglandin synthase I gene from mouse strain 129 genomic DNA was obtained. A targeting construct was made using 7.5kb of the genomic clone with an inserted bacterial neo gene and trailing herpes simplex virus TK gene. The construct was electroporated into embryonic stem cells and G418/gangcyclovir resistant clones were isolated. Homologously targeted ES cells were identified by Southern and PCR analysis. The targeted ES cells with a single allele of the PHS-1 disrupted have been injected into bastocysts and the blastocysts implanted. The births of chimeric and, subsequently, germline transmitting animals are awaited. These animals will be utilized to determine the role of PHS-1 in carcinogenesis.
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MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II
TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
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COMPARATIVE GENETIC TOXICOLOGY OF H.C. BLUE 1 AND H.C. BLUE 2
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