COEXISTENCE OF PEPTIDES AND NEUROTRANSMITTERS

钙结合蛋白钙视黄素(CR)的共存。和 酪氨酸羟化酶(TH)1合成中的限速酶 在大鼠黑质(SN)和腹侧 被盖区(VTA)。使用双标记免疫荧光，它 可以区分三种不同的细胞类型：细胞 仅CR(CR+)1细胞仅TH免疫阳性 (TH+)和两种蛋白都定位的细胞(CR+TH)。这个 SN致密区的CR+细胞数很低， 占细胞总数的不到10% 该区域的吻尾范围。TH+细胞与共定位细胞 (cr+th)约占总单元数的45% 算上了。相反，黑质网状结构中TH+细胞的数量为 吻部非常低(不到10%)，但在更多的部分数量上升 尾部切面(约20%)。平均CR+细胞 大约60%-70%在网状区。中的共址单元格 这一区域很少见(不到20%)。在VTA中， TH+细胞占细胞总数的近50%，而这一比例为15%-20% 对于CR+，共定位细胞为30-35%。当仅检查 含有CR的细胞，这些细胞中也有 在SN Comacta中包含TH约为80%，在 VTA。相反，含有CR的TH细胞的百分比更接近 在SN压缩中为50%，在VTA中为40%。协同本地化水平做到了 在SN中的这两种类型的单元格之间似乎没有区别 网状(接近50%)。 在钙诱导的多巴胺神经变性的背景下- 包含细胞，则在包含TH的细胞内存在CR可能是 被认为可能具有神经保护作用。因此，CR中的更改应该 被认为是帕金森氏症患者。

The coexistence of calretinin (CR), a calcium binding protein. and tyrosine hydroxylase (TH)1 the rate.limiting enzyme in the synthesis of dopamine, was studied in the rat substantia nigra (SN) and ventral tegmental area (VTA). Using double-labelling immunofluorescence, it was possible to distinguish three distinct cell types: cells immunoreactive for CR only (CR+)1 cells immunoreactive for TH only (TH+) and cells in which both proteins were localized (CR+TH). The number of CR+ cells in the SN compacta region was very low, constituting less than 10% of the total cells counted throughout the rostro-caudal extent of the region. TH+ cells and colocalized cells (CR+TH) compromised approximately 45% each of the total number of cells counted. In contrast, the number of TH+ cells in the SN reticulate was very low in rostral sections (less than 10%) but rose in number in more caudal sections (to approximately 20%). CR+ cells averaged approximately 60-70% in the reticulate region. Colocalized cells in this area were infrequent (less than 20%). In the VTA, the number of TH+ cells was nearly 50% of total cells counted, compared with 15-20% for CR+ and 30-35% for colocalized cells. When examining only the cells which contained CR, the percent of these cells which also contained TH was approximately 80% in the SN compacta and 65% in the VTA. In contrast, the percent of TH cells which contained CR was closer to 50% in the SN compacta and 40% in the VTA. Colocalization levels did not appear to differ between these two types of cells in the SN reticulate (close to 50%). Taken in the context of calcium-induced neurodegeneration of dopamine- containing cells, the presence of CR within cells containing TH may be considered as possibly neuroprotective. As such, changes in CR should be contemplated in Parkinson's disease.