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GENERATION OF MONOCLONALS TO HTLV II

GENERATION OF MONOCLONALS TO HTLV II
HTLV II 单克隆抗体的生成
批准号：
3792591
负责人：
M RUTA
金额：
$0.0万
依托单位：
CENTER BIOLOGICS EVALUATION RESEARCH TRANSFUSION
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3792591
关键词：
antiviral antibody human T cell lymphotropic virus type 2 hybridomas laboratory mouse monoclonal antibody western blottings

项目摘要

项目成果

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相关文献

中文摘要
翻译
我们正在生产针对HTLV II病毒蛋白的单克隆抗体
英文摘要
We are generating monoclonal antibodies to HTLV II viral proteins for use in Western blot and RIPA analysis of HTLV II infected cells. Mice were immunized with detergent disrupted HTLV II. Following repeated boosts, the mice were bled and the polyclonal sera tested on HTLV I and HTLV II Western Blot for reactivity. The mice were sacrificed and spleen cells fused with a fusion partner to generate hybridoma cell lines. 26 cell lines were cloned and chosen for study based on the reactivity of the cell supernatant on HTLV I and HTLV II ELISA assays. Preliminary results show reactivity of supernatant from 8 clones with a band that appears to be the HTLV II envelope glycoprotein on Western blot. The supernatants will be further characterized to determine their reactivities.
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会议论文
CHARACTERIZATION OF A NOVEL HTLV-II CELL LINE
HTLV-II ELISA AND WESTERN BLOTS
FGF RECEPTOR EXPRESSION IN TUMORS
SEROLOGIC MARKERS OF HTLV-I/II INFECTION