INTRACELLULAR ANTIBODY-MEDIATED VIRUS NEUTRALIZATION

许多针对病毒核心蛋白或非结构蛋白的抗体 有能力阻止病毒感染，如果它们被引入 病毒感染细胞的胞浆。抗体认识的最新进展 折叠表明抗体具有正确折叠和结合的能力 在胞浆中表达的抗原。因此，细胞，或转基因 表达一种胞浆形式抗体的动物 具有病毒复制能力的人应能抵抗病毒感染。vbl.使用 电穿孔诱导抗体进入我们已鉴定的细胞 阻断流感病毒感染的单抗，并具有 开始从杂交瘤细胞中克隆相应的基因 创造对流感病毒感染具有抵抗力的动物。

Many antibodies specific for viral core proteins or non-structural proteins have the capacity to block viral infection if they are introduced into the cytosol of virus infected cells. Recent advance in understanding antibody folding indicate that antibodies have the ability to properly fold and bind antigen when expressed in the cytosol. Therefore, cells, or transgenic animals expressing a cytosolic form of an antibody capable of interfering with viral replication should be resistant to viral infection. Using electroporation to induce antibodies into cells we have identified monoclonal antibodies that block infection with influenza viruses, and have begun to clone the corresponding genes from hybridoma cells with the goal of creating animals resistant to influenza virus infection.