DETERMINATION OF URUSHIOL IN ALLERGENIC EXTRACTS--URUSHIOL STABILITY STUDIES

毒葛等植物致敏提取物中的活性成分 毒木属植物一般被命名为 “漆酚”，五烯基或十七烯基儿茶酚的混合物。 提取物 目前可用于口服给药或注射的制剂 植物油，例如杏仁油、玉米油或橄榄油。 漆酚化合物可氧化 到能够进行迈克尔加成反应的邻醌 亲核化合物。 其他漆酚分子上的烯烃位点 可作为亲核试剂，导致漆酚的低聚 存在于溶液中。 该项目的目标是：（a）开发 漆酚的定量分析方法，包括 B）开发样品制备技术， 从植物油基质中分离漆酚; c）开发方法， 测定氧化和低聚漆酚降解产物; d） 将漆酚降解与浓度、溶液类型等因素相关联 和储存条件。 漆酚样品已被物种化， 通过气相色谱法对三甲基甲硅烷基衍生物进行定量， 极性固定相OV-255。 已进行了验证研究， 该方法 采用硅胶柱的正相HPLC法， 研究了 用于在高（更高）温度下分离漆酚的程序 超过100微克/毫升）的浓度， 漆酚固相萃取分离方法的研究 在低浓度（低于10微克/毫升）的情况下， 初始 涉及通过凝胶渗透的尿酚低聚物形态的研究 已经进行了色谱分析。

The active constituents in allergenic extracts of Poison Ivy and other plants of the genus toxicodendron have been generically designated as "urushiol," a mixture of penta- or heptadecenyl catechols. Extracts currently available for oral administration or injection are formulated in plant oils, e.g. almond, corn or olive oil. Urushiol compounds may oxidize to orthoquinones which are capable of undergoing Michael-addition reactions with nucleophilic compounds. Olefinic sites on other urushiol molecules may function as nucleophiles, resulting in oligomerization of urushiols present in solution. Objectives of this project are: a) to develop methods for the quantitative analysis of urushiol, including speciation of individual compounds; b) to develop sample preparation techniques to isolate urushiol from plant oil matrices; c) to develop methodologies to determine oxidized and oligomerized urushiol degradation products; d) to correlate urushiol degradation with factors of concentration, solution type and storage conditions. Urushiol samples have been speciated and quantitated by gas chromatography of trimethylsilyl derivatives using a polar stationary phase, OV-255. Validation studies have been performed for this method. A normal phase HPLC method utilizing a silica column is to be investigated. Procedures for the isolation of urushiol at high (greater than 100 microgram/ml) concentrations from plant oils have been validated, development of a solid phase extraction procedure for isolation of urushiol at low (less than 10 microgram/ml) concentrations is in progress. Initial studies involving speciation of uroshiol oligomers by gel permeation chromatography have been performed.