SEROLOGICAL RESPONSE TO BORDETELLA PERTUSSIS ANTIGENS

人类对几种包膜相关蛋白的血清学反应和 免疫印迹法检测百日咳杆菌腺苷环化酶毒素 技巧。有培养的个体的血清识别的抗原- 百日咳确诊以及由三种疫苗免疫的婴儿的血清检测 常规全细胞百日咳疫苗的剂量包括63,000 Da 被证明与分枝杆菌热相关的抗原性蛋白质- 休克蛋白及其与伴侣蛋白家族的结构关系 参与蛋白质折叠和组装。B.百日咳脂低聚糖 也被某些血清中的抗体识别。29,000 Da 物种与恢复期个体的血清发生反应，而91,000Da 物种与接种疫苗的个体的血清发生反应。抗病毒抗体 腺苷环化酶毒素常见于诊断为 然而，这些抗体在患有百日咳的儿童中很少发现 接种了三剂百白破疫苗。 从新生儿(脐带血)和相应的母亲血清中提取血清 检查波氏杆菌腺苷环化酶抗体。类似水平的 在每个血清样本中都发现了抗原特异性抗体(Ig G亚型) 一对给定的新生儿-母体提示抗腺苷酸抗体 环化酶通过胎盘被动转移。这些抗体 可能是母亲以前接触过百日咳杆菌而引起的 或通过母亲接种百日咳疫苗，因为所有四个 目前在美国使用的百日咳疫苗能够在 小鼠抗体与腺苷环化酶反应。或者，这些 抗体可能是由母亲暴露在交叉反应中引起的 从所有新生儿血清中发现的大肠杆菌a-溶血素等抗原 含有与这种抗原反应的抗体。腺苷酸类抗体 此前已有研究表明，环化酶可被动保护小鼠免受 挑战百日咳杆菌。因此，存在这样一种可能性，即 抗体可能有助于保护新生儿免受百日咳的侵袭。

The human serological response to several envelope-associated proteins and adenylate cyclase toxin of B. pertussis was examined using immunoblot techniques. Antigens recognized by sera from individuals with culture- confirmed pertussis as well as by sera from infants immunized with three doses of conventional whole-cell pertussis vaccine included a 63,000 Da protein that was shown to be antigenically related to a mycobacterial heat- shock protein and structurally related to the chaperonin family of proteins involved in protein folding and assembly. B. pertussis lipooligosaccharide was also recognized by antibodies in certain of these sera. A 29,000 Da species reacted with sera from convalescent individuals whereas a 91,000 Da species reacted with sera from vaccinated individuals. Antibodies to adenylate cyclase toxin were common in sera from individuals diagnosed with pertussis, however these antibodies were rarely found in children who had received three doses of DTP vaccine. Sera from neonates (cord blood) and corresponding maternal sera were examined for antibodies to Bordetella adenylate cyclase. Similar levels of antigen specific antibodies (IgG isotype) were found in each serum sample of a given neonate-maternal pair suggesting that antibodies to adenylate cyclase are passively transferred through the placenta. These antibodies may have been elicited by previous exposure of the mothers to B. pertussis or by vaccination of the mother with pertussis vaccine since all four pertussis vaccines currently in use in the US were capable of eliciting in mice antibodies reactive with adenylate cyclase. Alternatively, these antibodies may have been elicited by exposure of mothers to cross-reactive antigens such as E. coli a-hemolysin since all of the neonatal sera contained antibodies reactive with this antigen. Antibodies to adenylate cyclase have been previously shown to passively protect mice against challenge with B. pertussis. Therefore the possibility exists that these antibodies may help protect the newborn child against pertussis.