BLASTOCYSTIS HOMINIS--AXENIZATION OF STRAINS DEP. ON MIXED GROWTH BACTERIA

人芽囊原虫自身需要伴随细菌的生长 成长。有几个菌株已经在没有活体的情况下成功培养 细菌(无菌生长)经过一段时间的适应，具有较重的 抗生素的存在会逐渐消除细菌的种类。但这件事 是很罕见的。大多数轴心化的尝试都没有成功。B。人是这样的。 通常不会摄取细菌作为食物，但取决于产生的代谢物 是细菌造成的。 进展：消除大多数细菌和真菌的能力 B.人类的文化现在相当有把握。的补充用法 头孢唑酮、阿米卡星和红霉素的配伍不成功 对人芽孢杆菌是有毒的。头孢唑酮和万古霉素联合使用， 在1000兆克/毫升的最终浓度下，成功地消除了 残留类杆菌，但不是相关的支原体。 氨曲霉素成功去除了真菌和酵母菌。 支原体的种类可能来自使用的马血清。这种有机体 即使充分使用了两种抗生素鸡尾酒(五种)，也没有被消除 抗生素)。使用更放心的、不含支原体的马血清，来自 HyClone和Gibco可能会回答这个问题。我们的信念依然坚定， 然而，支原体来自人类的胃肠道。

Blastocystis hominis requires concomitant growth of bacteria for its own growth. A few strains have been successfully grown in the absence of live bacteria (axenic growth) after a period of adaptation, with heavy antibiotic presence to gradually eliminate the bacteria species. But this is rare. Most attempts at axenization are unsuccessful. B. hominis does not usually ingest bacteria for food, but depends on metabolites generated by bacteria. Progress: The ability to eliminate bacteria and fungi from the majority of B. hominis cultures is now fairly assured. The supplemental use of Ceftizoxime, Amikacin and Erythromycin was not successful, as the mixture was toxic to B. hominis. The use of Ceftizoxime and Vancomycin together, at 1000 meg/ml each, final concentration, was successful in eliminating residual Bacteroides species, but not the associated Mycoplasma. Amplutericin successfully removed fungus and yeast. The Mycoplasma species may derive from the horse serum used. This organism was not eliminated, even with full use of both antibiotic cocktails (five antibiotics). The use of more assured, Mycoplasma free horse sera, from Hyclone and Gibco, may answer this question. Our belief is still strong, however, that the Mycoplasm derives from the human GI tract.