BIOLOGY OF HUMAN AIDS RETROVIRUS

这项工作的目标一直是开发一种敏感的定量 人类免疫缺陷病毒(HIV)的体外定位免疫分析 以及感染艾滋病毒的细胞。这是通过将 将人CD4基因导入人细胞系。具有高CD4值的克隆 然后将表达作为HIV感染的目标，这是 用间接法检测HIV特异性抗体 免疫荧光法或免疫过氧化物酶法。化验结果是 通过使用针对HIV的单抗显示为HIV特异性 对于HIV包膜或核心蛋白；然而，多克隆人抗人 HIV血清也有效。这种化验方法非常灵敏， 能够检测到从中国分离的大多数野生型艾滋病毒毒株 病人。 未来的实验目标是使用这种分析方法来研究艾滋病毒 在艾滋病患者细胞中的表达。

The goal of this work has been to develop a sensitive quantitative in vitro focal immunoassay for human immunodeficiency virus (HIV) and HIV-infected cells. This has been approached by inserting the human CD4 gene into human cell lines. Clones with high CD4 expression were then used as targets for HIV infection which was detected by HIV-specific antibodies with indirect immunofluorescence or immunoperoxidase methods. The assay was shown to be HIV-specific by using monoclonal antibodies specific for HIV envelope or core proteins; however, polyclonal human anti- HIV serum was also effective. The assay was highly sensitive, capable of detecting most wild-type HIV strains isolated from patients. Future experiments are aimed at using the assay to study HIV expression in cells of AIDS patients.