EFFECT OF CYTOKINES IN HOST DEFENSE AND INFLAMMATION

已有研究开始探索细胞因子的重要性 吞噬细胞功能。慢性萎缩性胃炎患者吞噬细胞的研究 接受干扰素伽马或白介素2治疗的艾滋病患者表示 接受干扰素伽马治疗的患者明显较少 细菌感染比那些接受白介素2的人更多。我们也 研究了干扰素对中性粒细胞和单核细胞的影响。 来自儿童慢性肉芽肿性疾病的患者 (CGD)。干扰素-γ纠正异常氧化 许多CGD患者吞噬细胞在体外和体内的代谢 体内，这种药物被证明也修复了缺陷。在……上面 其他研究表明，干扰素-伽玛在慢性粒细胞白血病中的作用 高免疫球蛋白E与反复感染综合征 表明在体外和体内干扰素γ下调 B细胞合成免疫球蛋白E，而体内干扰素γ降低 本综合征患者IgE升高，趋于正常。干扰素-伽马是 在体外模型中被证明能导致单核细胞多核细胞的形成 肉芽肿的形成，通过一系列涉及 白介素L和肿瘤坏死因子。后一种影响可能 在体外可通过使用皮质类固醇来阻断。在其他研究中 在单核巨噬细胞的生长过程中，确定了一种技术 用于培养不转化的人巨噬细胞系。 干扰素-γ对单核细胞多核细胞影响的研究 形成表明人类巨噬细胞产生丰富的 脑脊液-L的含量，这可能与另一项观察有关 在适当的条件下，人类单核细胞发生复制 体外培养时使用“条件”培养液，富含脑脊液-L。

Studies have been initiated to explore the importance of cytokines in phagocyte function. Studies of phagocytes from patients with AIDS who were receiving interferon gamma or interleukin-2 indicated that patients receiving interferon gamma had markedly fewer bacterial infections than those receiving interleukin-2. We also studied the effect of interferon gamma on neutrophils and monocytes from patients with chronic granulomatous diseases of childhood (CGD). Interferon gamma corrected the abnormal oxidative metabolism in the phagocytes of many CGD patients in vitro and in vivo the drug was shown to reconstitute the defect as well. On other studies the effect of interferon gamma in the Hyperimmunoglobulin E and Recurrent Infections (Job's) Syndrome indicate that in vitro and in vivo interferon gamma down regulates B cell synthesis of IgE while in vivo interferon gamma lowers the elevated IgE in this syndrome towards normal. Interferon gamma was shown to cause monocyte polykaryon formation, in an in vitro model of granuloma formation, through a cascade of events involving interleukin-l and tumor necrosis factor. The latter effects could be blocked in vitro by use of corticosteroids. In other studies of the growth of mononuclear phagocytes, a technique was determined for developing human macrophage cell lines without transformation. Studies of the effect of interferon gamma on monocyte polykaryon formation demonstrated that human macrophages produce copious amounts of CSF-l and this may relate to an additional observation that under appropriate conditions human monocyte replication occurs in vitro when using "conditioned" growth media, rich in CSF-l.