COMPUTER SUPPORT FOR MOLECULAR SEQUENCING AND GENETIC MAPPING

计算机系统实验室（CSL）集成了实验室采购和 受体生物化学和分子生物学数据处理计算机系统 生物学分支（RBMB），NINDS。 七台自动DNA测序仪产生168个 序列片段或约60，000个碱基的序列。 我们有 指定、安装和管理计算机设施， 分析和存档大量的DNA序列数据。 在1992财政年度，CSL提供了系统管理、编程和数据库支持 cDNA项目，部分由能源部资助， 疾病控制，旨在确定和描述近30，000 在人脑中表达的基因，产生表达序列标签 从人脑cDNA中以大约100 templates/day. 已经发表和提交了6，000多个EST序列 基因库 同样，天花项目的目标， 与能源部疾病控制中心（DECDC）合作， 完成170千碱基的天花基因组测序， 1993年12月被销毁。 基因组测序和组装几乎是 目前正在对完成的序列进行分析。 系统管理协助包括为此提供日常业务支助 大批量生产系统、系统规范和启动 备份程序，以及安装和测试商业和公共 域软件。 一套程序被创建来批量处理EST， 数据库搜索 移植了一个蛋白质库基序比较程序 CSL并行计算机 CSL正在购买一个特殊用途的序列组装和分析系统 基于高速文本搜索计算引擎， 作为连接到NIH的共享资源， 网络 在1992财年，重点继续从序列组装转向 序列分析和数据存档/检索。 CSL将继续 专注于处理和归档超大卷的方法 序列数据，并帮助通过这一点开发技术， 该项目提供给NIH的其他研究人员。

Computer Systems Laboratory (CSL) has integrated laboratory acquisition and data processing computer systems in the Receptor Biochemistry and Molecular Biology Branch (RBMB), NINDS. Seven automated DNA sequencers produce 168 sequence fragments or about 60,000 bases of sequence per day. We have specified, installed and administered the computer facilities used for analyzing and archiving this large volume of DNA sequence data. In FY92 CSL provided system management, programming and database support for the cDNA Project, funded in part by the Department of Energy Center for Disease Control, that seeks to identify and characterize the nearly 30,000 genes expressed in the human brain, producing expressed sequence tags (ESTs) from human brain cDNAs at the rate of approximately 100 templates/day. Over 6,000 EST sequences have been published and submitted to GenBank. Similarly, the goal of the smallpox project, a collaboration with the Department of Energy Centers for Disease Control (DECDC), is to sequence completely the 170 kilo-base smallpox genome prior to its destruction in December, 1993. Genome sequencing and assembly is nearly complete and analysis of the finished sequence is underway. System management assistance included daily operations support for this high-volume production system, specification and initiation of system backup procedures, and installation and testing of commercial and public domain software. A set of programs was created to batch process ESTs for database searching. A protein library motif comparison program was ported to the CSL parallel computer. CSL is purchasing a special purpose sequence assembly and analysis system based on a high speed text search computational engine to be made available to the NIH intramural community as a shared resource connected to the NIH network. During FY92, emphasis has continued to shift from sequence assembly to sequence analysis and data archival/retrieval. CSL will continue to concentrate on methods for processing and archiving extremely large volumes of sequence data, and help to make technologies developed through this project available to other investigators at NIH.