MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA

我们致力于研究真核生物的结构和调控。 基因，以果蝇为实验对象。虽然我们来自 不同背景(遗传学、分子生物学、发育生物学、 人口遗传学、细胞生物学)，我们为了我们的共同利益而相交： 利用遗传上最有利的高等生物--果蝇和 目前的分子和细胞生物学技术，以阐明 高等的基因在发育过程中被组织、进化和调节 在细胞周期中。事实上，我们集团的一个优势是它 代表了相辅相成但又重叠的方法。在…的背景下 该计划项目，我们打算： (A)从遗传学角度测试转录因子的体内功能 推测参与了绒毛膜基因的发育调节，以及 使用增强子分离参与绒毛膜调节的其他成分 诱捕法。 (B)研究和操纵流浪汉流动要素，包括 转座酶转录本的特性及其作用机制 胚系中元素的转位；开发新的转化 向量和程序。 (C)描述十足瘫痪基因的DNA多态和进化 在黑腹夜蛾和拟黑腹夜蛾种群中，获得了第一个 关于氨基酸序列、消息结构的选择性限制的数据 和控制基本形态发生的基因的顺式调控元件 流程。 (D)使用遗传学方法了解胚胎肌肉的控制 细胞承诺和分化，重点是编码NAU基因 基本结构域和螺旋环状结构域类似于脊椎动物的肌源性结构域 监管因素。 (E)研究其产品的体外性质和体内功能 两个基因，Ned和nod，属于kinesin超家族和 微管在减数分裂和有丝分裂纺锤体中的运动。

We are involved in studying the structure and regulation of eukaryotic genes, using Drosophila as an experimental object. Although we come from diverse background (genetics, molecular biology, developmental biology, population genetics, cell biology), we intersect in our common interest: to use the genetically most favorable higher organism, Drosophila, and the current techniques of molecular and cellular biology, to illuminate how the genes of higher are organized, evolve, and are regulated during development and during the cell cycle. Indeed, a strength of our group is that it represents complementary and yet overlapping approaches. In the context of the program project, we intend to: (a) Test by genetics the in vivo function of transcription factors putatively involved in the developmental regulation of chorion genes, and isolate additional components involved in chorion regulation using enhancer trap method. (b) Study and manipulate the hobo mobile elements, including characterization of the transposase transcript and the mechanism of transposition of the element in the germ line; develop novel transformation vector and procedures. (c) Characterize DNA polymorphism and evolution of the decapentaplegic gene in populations of D. melanogaster and D. pseudoobscura, to obtain the first data on selective constraints on amino acid sequences, message structure and cis-regulatory elements for a gene controlling basic morphogenetic processes. (d) Use genetic approaches to understanding the control of embryonic muscle cell commitment and differentiation, focusing on the nau gene that encodes basic and helix-loop-lelix domains similar to those of vertebrate myogenic regulatory factors. (e) Study the in vitro properties and in vivo function of the products of two genes, ned and nod, which belong to the kinesin superfamily and movements of microtubules in the meiotic and mitotic spindle apparatus.