COLLABORATIVE PROJECTS IN ENVIRONMENTAL HEALTH SCIENCES

环境健康科学领域的合作项目包括 质谱学工作组与其他机构合作的项目 团体，无论是研究所内还是研究所外，都要解决相互之间的问题 利息。这些项目的主要关注点是1)结构 结构未知的生物化合物的测定，2) 识别和/或确认生物途径和3) 生物化学结构测定策略的发展 重要的化合物。 目前正在调查的主要项目包括：1)结构 血清中低相对分子质量化合物肝生成素B的测定 刺激肝细胞DNA合成，并被假设为 由异类化合物模仿的原型分子，如苯巴比妥， 导致肝细胞肥大和增殖；2)结构 呼吸道感染细胞表达的小肽的测定 使细胞被T细胞识别为被感染的病毒(a HIV感染模型)和由癌症表达的小肽 3)LC/EPR/MS联用技术的发展 自旋俘获自由基的鉴定。后一个项目涉及 化学产生的自由基的结构测定 在酶过程中作为中间体产生的自由基 生物系统，以及4)血液中苯乙烯和 注射或吸入染毒大鼠体内的环氧苯乙烷。我们 也在开发技术，使我们能够检测血红蛋白 来自暴露大鼠的环氧苯乙烷的血红蛋白加合物用作 暴露的标记。

Collaborative projects in the environmental health sciences include those projects in which the mass spectrometry work-group collaborates with other groups, both within and without the institute to solve problems of mutual interest. The major focuses of these projects are 1) the structure determination of biological compounds of unknown structure, 2) identification and/or confirmation of biological pathways and 3) development of strategies for the structure determination of biologically important compounds. Major projects currently under investigation include: 1) the structure determination of hepatopoietin B, a low molecular weight compound in serum that stimulates hepatocyte DNA synthesis and is hypothesized to be the prototype molecule mimicked by xenobiotic compounds, such phenobarbital, that cause hepatocyte hypertrophy and proliferation; 2) the structure determination of the small peptides expressed by cells infected with flue virus that cause the cell to be recognized by T-cells as being infected (a model of HIV infection) and of the small peptides expressed by cancer cells; 3) the development of combined LC/EPR/MS techniques for the identification of spin-trapped free radicals. This latter project involves the structure determination of chemically produced free-radicals and of free-radicals produced as intermediates in enzymatic processes in biological systems, and 4) determination of blood levels of styrene and styrene oxide in rats exposed to styrene by injection or inhalation. We are also developing techniques that will allow us to examine hemoglobin from the exposed rats for hemoglobin adducts of styrene oxide to be used as a marker for exposure.