DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDYING BIOCHEMICAL REACTIONS

关于开发各种评估仪器的工作 作为血液替代品的血红蛋白衍生品已经在一个 随着LAIR试点工厂的上线，速度加快 可以提供大量的蛋白质。氧平衡曲线 该仪器现正在人体血红蛋白样本上进行测试和量热 一氧化碳、二氧化碳和氯化物与HbAo和DBBF结合的测量- HB产品正在进行中。脉冲加速流动装置几乎 准备好行动了。在评估中具有相当重要的意义 产品是否适合注射入人体取决于产品的纯度 材料，氧平衡曲线的精确知识和 蛋白质对其各种效应物的反应，如氯化物，pH， 温度、磷酸盐、二氧化碳以及氧气浓度。装订 氧和一氧化碳在存在和不存在的情况下的等温线 这些效应器在压力计和光学上都被确定为 在微量热计中也是如此。正在确定二氧化碳结合曲线 量热法和C_(13)核磁共振。血红蛋白的物理化学有 过去因缺乏适当的标准状态而遭受痛苦， 开始自由能计算。关于等离子的这些研究 蛋白质，即在没有所有效应器的情况下，将允许这样的标准 待建立的国家。

Work on the development of instrumentation for the evaluation of various hemoglobin derivatives proposed as a blood substitute has proceeded at an accelerated rate with the coming on line of the pilot plant at LAIR which can furnish large amounts of the protein. The oxygen equilibrium curve apparatus is now being tested on human hemoglobin samples and calorimetric measurements of the binding of CO,CO2, and chloride to HbAo and the DBBF- HB product are underway. The pulsed accelerated flow apparatus is nearly ready for operation. Of considerable importance in evaluating the product's suitability for injection into humans is the purity of the material, a precise knowledge of the oxygen equilibrium curve and the protein's response to its various effectors, i.e. chloride, pH, temperature, phosphate, CO2, as well as oxygen concentration. Binding isotherms of oxygen and carbon monoxide in the presence and absence of these effectors are being determined both manometrically and optically as well as in the microcalorimeter. CO2 binding curves are being determined calorimetrically and by C13 NMR. The physical chemistry of hemoglobin has suffered in the past from the lack of a suitable standard state from which to start the free energy calculations. These studies on the isoionic protein, i.e. in the absence of all effectors, will allow such a standard state to be established.